Rabbit anti-Rat IgA/IgG/IgM Secondary Antibody [FITC]



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Product Discontinued
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Rabbit anti-Rat IgA/IgG/IgM Secondary Antibody [FITC] Summary

The antibody reacts with the Rat IgG antigen.
Antibodies were raised in rabbit to rat IgG, IgM and IgA. Specific antibodies were absorpted by incubation with Sepharose-bound rat immunoglobulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized rat immunoglobulins a minimum of 65% protein bound. The purified polyclonal was labeled with fluorescein isothiocyanate isomer I followed by gel-filtation and ion-exchange chromatography to clear unbound conjugate. F/P ratio: 3-5 mol Fluorescein per 1 mol rabbit IgG. Low background fluorescence of normal tissue in contrast to specific staining of sections treated with monoclonal rat anti-human keratin 18 antibodies. No cross reactivity with albumine fractions of rat serum. No cross reactivity with albumine fractions of animal species.
Immunogen affinity purified

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
FITC (A=495, E=519)
No Preservative
Immunogen affinity purified


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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IgA/IgG/IgM NBP1-42104

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