Rabbit anti-Rat IgA/IgG/IgM Secondary Antibody [FITC]



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Rabbit anti-Rat IgA/IgG/IgM Secondary Antibody [FITC] Summary

The antibody reacts with the Rat IgG antigen.
Antibodies were raised in rabbit to rat IgG, IgM and IgA. Specific antibodies were absorpted by incubation with Sepharose-bound rat immunoglobulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized rat immunoglobulins a minimum of 65% protein bound. The purified polyclonal was labeled with fluorescein isothiocyanate isomer I followed by gel-filtation and ion-exchange chromatography to clear unbound conjugate. F/P ratio: 3-5 mol Fluorescein per 1 mol rabbit IgG. Low background fluorescence of normal tissue in contrast to specific staining of sections treated with monoclonal rat anti-human keratin 18 antibodies. No cross reactivity with albumine fractions of rat serum. No cross reactivity with albumine fractions of animal species.
Immunogen affinity purified

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
50% glycerol with 0.1 M NaCl, 0.01M sodium phosphate at pH 7.5
No Preservative
Immunogen affinity purified


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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