Rabbit anti-Human IgA Secondary Antibody [Biotin]


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Product Details

Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
LYOPH mg/ml

Order Details

Rabbit anti-Human IgA Secondary Antibody [Biotin] Summary

Human IgA alpha heavy chain
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Human IgA and Human Serum. No reaction was observed against Human IgG or IgM. Specificity was confirmed at less than 1% against other human heavy or light chain isotypes by ELISA.
Immunogen affinity purified

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Biotin (A=N/A, E=N/A)
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01% Thimerosal
LYOPH mg/ml
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 1.0 ml deionized water.


  • Western Blot 1:2000 - 1:10000
  • ELISA 1:20000 - 1:100000
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin
Application Notes
This product has been assayed against 1.0 ug of Human IgA in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:10,000 - 1:100,000 of the reconstitution concentration is suggested for this product.
Readout System


Label Molecular Weight: 455, Restore with 1.0 mL of deionized water (or equivalent).

Alternate Names for Rabbit anti-Human IgA Secondary Antibody [Biotin]

  • Arc-1
  • cadherin 1, E-cadherin (epithelial)
  • cadherin 1, type 1, E-cadherin (epithelial)
  • cadherin-1
  • calcium-dependent adhesion protein, epithelial
  • CAM 120/80
  • CD324 antigen
  • CD324
  • CDHE
  • cell-CAM 120/80
  • ECAD
  • E-cadherin
  • Epithelial cadherin
  • LCAM
  • UVOE-Cadherin
  • uvomorulin


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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WB Video Protocol

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Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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