Recombinant Mouse IFN-gamma Protein

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-43203
    • Availability
      Product Discontinued

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Recombinant Mouse IFN-gamma Protein Summary

Description
A partial protein corresponding to IFNG.
Preparation
Method
E. coli expressed amino acids His 23-Cys 155 of mouse IFN-g (accession # NM_008337).
Details of Functionality
Measured by mouse L929/EMCV cell protection assay. The ED50 measured in a L929 protection is typically 100 pg/ml, corresponding to a specific activity of approximately 1x10^7Units/mg.
Protein/Peptide Type
Partial Recombinant Protein
Gene
IFNG
Endotoxin Note
Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Applications/Dilutions

Application Notes
This reagent has been tested in bioassays using the mouse fibroblast cell line L929. The ED50 measured in a L929 protection is typically 100 pg/ml, corresponding to a specific activity of approximately 1x10^7Units/mg.

Reactivity Notes

This protein was made to the mouse sequence.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sterile 10 mM NaH2PO4, 150 mM NaCl, 1 mM DTT, with 0.5% BSA. 0.22 um filtered.
Preservative
0.09% Sodium Azide

Alternate Names for Recombinant Mouse IFN-gamma Protein

  • IFG
  • IFI
  • IFNG
  • IFNgamma
  • IFN-gamma
  • Immune interferon
  • interferon gamma
  • interferon, gamma

Background

Mouse IFN-gamma is a ~20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN-gamma, in synergy with other cytokines such as TNF-alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN-gamma does not affect its biological activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol IFNG
Entrez