Recombinant Human IFN-gamma Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications IA
Concentration
0.5 mg/ml

Order Details

Recombinant Human IFN-gamma Protein Summary

Description
Recombinant protein corresponding to Gln24-Gln166 of mature human IFN gamma. Accession #: NM_000619.

Source: E.coli

Details of Functionality
This recombinant human IFN-gamma; has been tested in bioassays using mouse cell line A549. The ED50 measured in A549 protection is typically 175 pg/ml, corresponding to a specific activity of approximately 5.7 x 10^6 Units/mg.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
IFNG
Purity
>95% pure by SDS-PAGE
Endotoxin Note
Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Applications/Dilutions

Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline, pH 7.2
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human IFN-gamma Protein

  • IFG
  • IFI
  • IFNG
  • IFNgamma
  • IFN-gamma
  • Immune interferon
  • interferon gamma
  • interferon, gamma

Background

Human IFN-gamma is an ~20 kDa factor produced by activated T, B and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-gamma in synergy with other cytokines, such as TNF-alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN-gamma does not affect its biological activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol IFNG
Entrez