Recombinant Mouse IFN-alpha 4 Protein

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-42903
    • Availability
      Product Discontinued

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Recombinant Mouse IFN-alpha 4 Protein Summary

Description
A partial protein corresponding to IFNA4.
Preparation
Method
E. coli expressed amino acids Cys25-Glu186 of mature mouse IFNa4 (accession # NM_010504).
Details of Functionality
The recombinant mouse IFN-alpha 4 protein has been tested for inhibition of the cytopathic effect of EMC virus on L929 cells. The ED50 for this effect is typically 40 pg/ml, corresponding to a specific activity of 2 x 10^7 U/mg.
Protein/Peptide Type
Partial Recombinant Protein
Gene
IFNA4
Endotoxin Note
Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Applications/Dilutions

Application Notes
The recombinant mouse IFN-α4 has been tested for inhibition of the cytopathic effect of EMC virus on L929 cells. The ED50 for this effect is typically 40 pg/ml, corresponding to a specific activity of 2 x 10^7U/mg.

Reactivity Notes

This protein was made to the mouse sequence.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sterile liquid; 20 mM Tris-HCl, 0.15 M NaCl, pH 7.5, 0.22 uM filtered.
Preservative
0.09% Sodium Azide

Notes

Purity: Greater than 98% as determined by SDS-PAGE

Alternate Names for Recombinant Mouse IFN-alpha 4 Protein

  • IFNA4
  • IFNalpha 4
  • IFN-alpha 4
  • IFN-alpha-4
  • IFN-alpha4a
  • INFA4
  • interferon alpha-4
  • Interferon alpha-4B
  • Interferon alpha-76
  • Interferon alpha-M1
  • interferon, alpha 4
  • MGC142200

Background

IFN-alpha4 is a type I interferon, previously known as B-cell interferon, leukocyte interferon, lymphoblast interferon, and pH2-stable interferon. IFN-alpha4 is one of at least 23 different known variants of IFN-alpha. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. IFN-alpha forms are produced by monocytes/macrophages, lymphoblastoid cells, fibroblasts, and a number of different cell types following induction by viruses, nucleic acids, glucocorticoid hormones, and low-molecular weight substances (n-butyrate, 5-bromodeoxyuridine). IFN-alpha4 demonstrates antiviral, antiparasitic,and antiproliferative activities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol IFNA4
Entrez