IFN-alpha 2 Antibody - BSA Free

Western Blot: Recombinant human IFNa2b as antigen. IgY dilution: 1:2000; Goat anti-IgY Fc-HRP: 1: 1000. Colorimetric method for signal development. ( anti-IFNA2 )

ELISA: Interferon alpha 2b Antibody [NB100-75556] - Recombinant protein as test antigen. Affinity-Purified anti- IFNa2b IgY as primary antibody (0.1 ug/ml), and Goat anti-IgY-HRP as 2nd antibody. ( anti-IFNA2 )

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: BSA Free

IFN-alpha 2 Antibody - BSA Free Summary

• Description: Novus Biologicals Chicken IFN-alpha 2 Antibody - BSA Free (NB100-75556) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this antibody was a sequence from 25a.a. to 186a.a. from Interferon alpha 2b(a 188a.a. long protein)
• Localization: Secreted
• Specificity: Interferon alpha 2b
• Clonality: Polyclonal
• Host: Chicken
• Gene: IFNA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:200-1:500
  • Western Blot 1:200-1:500
• Application Notes: ELISA: Recombinant protein as test antigen. Affinity-Purified anti- IFNa2b IgY as primary antibody (0.1 ug/ml). and Goat anti-IgY-HRP as 2nd antibody.
• Control:

  IFN-alpha 2 Overexpression Lysate

Reactivity Notes

This antibody is reactive against Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for IFN-alpha 2 Antibody - BSA Free

• alpha-2a interferon
• Ifa2
• IFNA
• IFNA2
• IFNA2a
• IFNA2b
• IFNA2c
• IFNAA
• IFNalpha 2
• IFN-alpha 2
• IFN-alpha-2
• IFN-alphaA
• INFA2
• interferon alpha 2b
• interferon alpha A
• interferon alpha-2
• Interferon alpha-A
• interferon, alpha 2
• LeIF A
• MGC125764
• MGC125765

Background

Interferon alpha 2b is one of the known 23 different variants of Interferon alpha 2b. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end. Interferon alpha 2b is a potent immune modulator. It can either inhibit or enhance immune cell activity within the tightly regulated microenvironment of inflammation, depending upon the concentration of the cytokine and the activation stage of the cell. Interferon alpha 2B is currently used as an anticancer drug due to it's antiproliferative activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IFNA2
• Entrez:
  • 3440(Human)
• Uniprot:
  • P01563()