Recombinant biologically active Interferon alpha protein.
Protein quantitation was carried out by two independent methods: 1.UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinctioncoefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a standard solution of IFN-alpha(2a) as a Reference Standard.
Details of Functionality
IFN-alpha 2a is fully biologically active when compared to standard.The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 2.7 x 10^8 IU/mg.
> 97 % pure by SDS-PAGE and HPLC
Less than 0.1 ng/ug (IEU/ug) of rHuIFN- 2a.
19.24 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
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