Iduronate 2-Sulfatase/IDS Antibody (4G2)

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Western Blot: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Iduronate 2 sulfatase monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Iduronate 2 sulfatase.
Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Human tonsil.
Flow Cytometry: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Iduronate 2 ...read more
Western Blot: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Iduronate 2 sulfatase (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Staining of paraffin-embedded Human Kidney tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Staining of paraffin-embedded Human liver tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Staining of paraffin-embedded Human lymphoma tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Staining of paraffin-embedded Human prostate tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Carcinoma of Human liver tissue.
Flow Cytometry: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Analysis of Hela cells, using anti-Iduronate 2 sulfatase antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Analysis of Jurkat cells, using anti-Iduronate 2 sulfatase antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, MkSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
4G2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Iduronate 2-Sulfatase/IDS Antibody (4G2) Summary

Immunogen
Full length human recombinant protein of human IDS(NP_000193) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
IDS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Iduronate 2-Sulfatase/IDS Antibody (4G2)

  • Alpha-L-iduronate sulfate sulfatase
  • EC 3.1.6.13
  • IDS
  • iduronate 2-sulfatase 14 kDa chain
  • iduronate 2-sulfatase 42 kDa chain
  • iduronate 2-sulfatase
  • idursulfase
  • MPS2
  • S
  • SIDS

Background

Iduronate-2-sulfatase is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this X-chromosome gene that result in enzymatic deficiency lead to the sex-linked Mucopolysaccharidosis Type II, also known as Hunter Syndrome. Iduronate-2-sulfatase has a strong sequence similarity with human arylsulfatases A, B, and C, and human glucosamine-6-sulfatase. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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