IDH3A Antibody (2F11)

Images

 
Western Blot: IDH3A Antibody (2F11) [NBP2-02158] Analysis of extracts (35ug) from 9 different cell lines by using anti-IDH3A monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: IDH3A Antibody (2F11) [NBP2-02158] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IDH3A.
Immunohistochemistry-Paraffin: IDH3A Antibody (2F11) [NBP2-02158] - Staining of paraffin-embedded liver tissue using anti-IDH3A mouse monoclonal antibody.
Flow Cytometry: IDH3A Antibody (2F11) [NBP2-02158] - Analysis of Jurkat cells, using anti-IDH3A antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Western Blot: IDH3A Antibody (2F11) [NBP2-02158] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDH3A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: IDH3A Antibody (2F11) [NBP2-02158] - Immunofluorescent staining of HepG2 cells using anti-IDH3A mouse monoclonal antibody.
Flow Cytometry: IDH3A Antibody (2F11) [NBP2-02158] - Analysis of Hela cells, using anti-IDH3A antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
2F11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

IDH3A Antibody (2F11) Summary

Immunogen
Full length human recombinant protein of human IDH3A (NP_005521) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
IDH3A
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for IDH3A Antibody (2F11)

  • EC 1.1.1
  • EC 1.1.1.41
  • H-IDH alpha
  • isocitrate dehydrogenase (NAD+) alpha chain
  • isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
  • isocitrate dehydrogenase 3 (NAD+) alpha
  • Isocitric dehydrogenase subunit alpha
  • NAD(+)-specific ICDH subunit alpha
  • NAD(H)-specific isocitrate dehydrogenase alpha subunit
  • NAD+-specific ICDH

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IDH3A
Entrez