ID3 Antibody (5C7)

Images

 
Western Blot: ID3 Antibody (5C7) [NBP2-02137] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug ...read more
Immunocytochemistry/ Immunofluorescence: ID3 Antibody (5C7) [NBP2-02137] Staining of COS7 cells transiently transfected by pCMV6-ENTRY ID3.
Immunohistochemistry-Paraffin: ID3 Antibody (5C7) [NBP2-02137] - Staining of paraffin-embedded Human thyroid tissue using anti-ID3 mouse monoclonal antibody.
Flow Cytometry: ID3 Antibody (5C7) [NBP2-02137] - Analysis of Jurkat cells, using anti-ID3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Immunohistochemistry-Paraffin: ID3 Antibody (5C7) [NBP2-02137] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ID3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ID3 Antibody (5C7) [NBP2-02137] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ID3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ID3 Antibody (5C7) [NBP2-02137] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ID3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ID3 Antibody (5C7) [NBP2-02137] - Staining of paraffin-embedded Human bladder tissue using anti-ID3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ID3 Antibody (5C7) [NBP2-02137] - Staining of paraffin-embedded Human breast tissue using anti-ID3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ID3 Antibody (5C7) [NBP2-02137] - Staining of paraffin-embedded Human colon tissue using anti-ID3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ID3 Antibody (5C7) [NBP2-02137] - Staining of paraffin-embedded Human endometrium tissue using anti-ID3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ID3 Antibody (5C7) [NBP2-02137] - Staining of paraffin-embedded Human Kidney tissue using anti-ID3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ID3 Antibody (5C7) [NBP2-02137] - Staining of paraffin-embedded Human liver tissue using anti-ID3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ID3 Antibody (5C7) [NBP2-02137] - Staining of paraffin-embedded Human lung tissue using anti-ID3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ID3 Antibody (5C7) [NBP2-02137] - Staining of paraffin-embedded Human Ovary tissue using anti-ID3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ID3 Antibody (5C7) [NBP2-02137] - Staining of paraffin-embedded Human pancreas tissue using anti-ID3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ID3 Antibody (5C7) [NBP2-02137] - Staining of paraffin-embedded Human prostate tissue using anti-ID3 mouse monoclonal antibody.
Flow Cytometry: ID3 Antibody (5C7) [NBP2-02137] - Analysis of Hela cells, using anti-ID3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-02137
    • Availability
      Product Discontinued

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ID3 Antibody (5C7) Summary

Immunogen
Full length human recombinant protein of human ID3 (NP_002158) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ID3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ID3 Antibody (5C7)

  • BHLHB25
  • bHLHb251R21
  • Class B basic helix-loop-helix protein 25
  • DNA-binding protein inhibitor ID-3
  • HEIR1
  • HEIR-1
  • Helix-loop-helix protein HEIR-1
  • ID-like protein inhibitor HLH 1R21
  • Inhibitor of DNA binding 3
  • inhibitor of DNA binding 3, dominant negative helix-loop-helix protein

Background

Members of the ID family of helix-loop-helix (HLH) proteins lack a basic DNA-binding domain and inhibit transcription through formation of nonfunctional dimers that are incapable of binding to DNA.[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ID3
Entrez