ID2 Antibody (9A8)

Images

 
Western Blot: ID2 Antibody (9A8) [NBP1-47802] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug ...read more
Immunocytochemistry/ Immunofluorescence: ID2 Antibody (9A8) [NBP1-47802] Staining of COS7 cells transiently transfected by pCMV6-ENTRY ID2.
Immunohistochemistry-Paraffin: ID2 Antibody (9A8) [NBP1-47802] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ID2 mouse monoclonal antibody.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-47802
    • Availability
      Product Discontinued

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ID2 Antibody (9A8) Summary

Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 1-134 of human ID2
Specificity
This antibody is specific for Homo sapiens inhibitor of DNA binding 2, dominant negative helix-loop-helix protein (ID2).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ID2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ID2 Antibody (9A8)

  • BHLHB26
  • bHLHb26cell growth-inhibiting gene 8
  • Class B basic helix-loop-helix protein 26
  • DNA-binding protein inhibitor ID2
  • DNA-binding protein inhibitor ID-2
  • GIG8
  • helix-loop-helix protein ID2
  • ID2
  • ID2A
  • ID2H
  • inhibitor of differentiation 2
  • Inhibitor of DNA binding 2
  • inhibitor of DNA binding 2, dominant negative helix-loop-helix protein
  • MGC26389

Background

The protein encoded by this gene belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene has been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ID2
Entrez