ICOS Antibody (C398.4A) [Biotin] Summary
Mouse T cell clone D10.G4.1
Protein A or G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Flow Cytometry 1:10-1:1000
The ISA-3 antibody has been tested by flow cytometric analysis of unstimulated and PHA-activated (2 days) human blood leukocytes. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
May react with Rhesus and Swine
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
0.09% Sodium Azide
Protein A or G purified
Alternate Names for ICOS Antibody (C398.4A) [Biotin]
- Activation-inducible lymphocyte immunomediatory molecule
- CD278 antigen
- inducible costimulator
- inducible T-cell costimulator
- inducible T-cell co-stimulator
The ISA-3 monoclonal antibody reacts with human ICOS (Inducible COStimulatory molecule), also known as H4, CRP-1 and AILIM. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. Human ICOS has a relative molecular mass of 55-60 kDa, composed of 27 kDa and 29 kDa chains. Human ICOS on activated T cells has potent costimulatory activity for T cell activation and is required for humoral immune responses, in particular for memory B cell and plasma cell generation. ICOS binds to its ligand, B7h/B7RP-1 expressed on activated APCs (antigen presenting cells) and on a number of inflamed peripheral tissues. Plate-bound ISA-3 is costimulatory for T cells and induces production of IL-4, IL-5, IL-10 and other cytokines, but not IL-2. ISA-3 has the same reactivity pattern and characteristics as F44. ISA-3 was generated against the human ICOS antigen. C398.4A, anti-mouse ICOS/H4, was shown to cross-react with human ICOS but binds to an epitope different from ISA-3. C398.4A stains activated cells brighter than ISA-3 however, it also exhibits higher staining of non-activated human peripheral blood or isolated PBMC. To achieve the brightest staining of ICOS on activated human T cells, please use or rather than.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
Customers Who Viewed This Item Also Viewed...
Applications: WB, Flow, CyTOF-ready
Applications: WB, AgAct
Applications: WB, IHC, IHC-P
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
Applications: Flow, IHC, CyTOF-ready, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Applications: WB, Flow, IHC, Block, CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta)
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu
Applications: WB, IHC
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
Applications: Flow, IHC, CyTOF-reported, ICC, Neut
Publications for ICOS Antibody (NBP1-43472) (0)
There are no publications for ICOS Antibody (NBP1-43472).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for ICOS Antibody (NBP1-43472) (0)
There are no reviews for ICOS Antibody (NBP1-43472).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
- 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for ICOS Antibody (NBP1-43472) (0)
Additional Array Products
Bioinformatics Tool for ICOS Antibody (NBP1-43472)
Discover related pathways, diseases and genes to ICOS Antibody (NBP1-43472). Need help?
Read the Bioinformatics Tool Guide
for instructions on using this tool.
Blogs on ICOS