ICOS Antibody (C398.4A)

Images

 
Flow Cytometry: ICOS Antibody (C398.4A) [NB100-77934] - Flow Cytometry: ICOS Antibody (ISA-3) [PE] [NBP1-43707] - See notes for image caption
Flow Cytometry: ICOS Antibody (C398.4A) [NB100-77934] - PHA-stimulated human peripheral blood lymphocytes (3 days) stained with purified C398.4A, followed by biotinylated anti-Armenian hamster IgG and Sav-PE
Flow Cytometry: ICOS Antibody (C398.4A) [NB100-77934] - Analysis using the PE conjugate of NB100-77934. Analysis of unstimulated and PHA-activated (2 days) human blood leukocytes using ICOS PE. This can be used at less ...read more
Flow Cytometry: ICOS Antibody (C398.4A) [NB100-77934] - Analysis using the Biotin conjugate of NB100-77934. Staining of 2-day unstimulated (left) and 2-day Anti-Human CD3/CD28 and activated (right) PBMC with Mouse IgG1 ...read more
Flow Cytometry: ICOS Antibody (C398.4A) [NB100-77934] - Analysis using the FITC conjugate of NB100-77934. Normal human peripheral blood cells were stimulated with Anti-Human CD3 Functional Grade Purified and Anti-Human ...read more
Flow Cytometry: ICOS Antibody (C398.4A) [NB100-77934] - Analysis using the FITC conjugate of NB100-77934. Staining of PHA-stimulated human peripheral blood lymphocytes (3 days) with C398.4A FITC.
Flow Cytometry: ICOS Antibody (C398.4A) [NB100-77934] - Analysis using the PE/Cy5 conjugate of NB100-77934. Staining of Con A-stimulated mouse spenocytes with Anti-Mouse/Rat CD278 (ICOS) FITC (left) and PE (right). ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-77934
    • Availability
      Product Discontinued

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ICOS Antibody (C398.4A) Summary

Immunogen
Mouse T cell clone D10.G4.1
Specificity
CD278 (C398.4A)
Isotype
IgG
Clonality
Monoclonal
Host
Armenian Hamster
Gene
ICOS
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 1.0 microgram per one million cells in 100 microliter volume. Immunohistochemistry-frozen sections. It is recommended that reagents be titrated for optimal performance in the particular application.

Reactivity Notes

May react with Rhesus and Swine

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for ICOS Antibody (C398.4A)

  • Activation-inducible lymphocyte immunomediatory molecule
  • AILIM
  • AILIMCD278
  • CD278 antigen
  • CD278
  • CRP-1
  • CVID1
  • ICOS
  • inducible costimulator
  • inducible T-cell costimulator
  • inducible T-cell co-stimulator
  • MGC39850

Background

The C398.4A antibody reacts with the human, mouse and rat 47-57 kD ICOS protein, also known as inducible costimulatory molecule and H4. This protein is homologous to the CD28/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes. It is able to costimulate T cells proliferation. In addition, ICOS is involved in humoral immune responses (B cell germinal center formation). The ICOS ligand is B7h/B7RP-1 or B7-H2. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development. The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ICOS
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