Ibuprofen Antibody

• Reactivity: All-NA
• Applications: ELISA, AP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Ibuprofen Antibody Summary

• Description: Store at 4C or -20 C. The working antibody solution is stable for at least 7 days at 4C. Precautions should be taken for storage for longer periods. Problems of long-term stability may occur with highly diluted solutions. For storage purposes in solution, the addition of sodium azide to 0.02 % is advised with the appropriate precautions of use.
• Immunogen: BSA-Ibuprofen derivative conjugate
• Specificity: Target: Ibuprofen, CAS no.: 15687-27-1, Solubility: Organic solvents, PEGs.
  
  The specificity of the antibody was determined by measuring the cross-reactivity in ELISA for a range of compounds.
  
  Compound tested: % Cross- reactivity
  
  Ibuprofen: 100%
  
  Bezafibrate: 0.4%
  
  Gemfibrozil: 0.2%
  
  Bisphenol A: 0.6%
  
  Carazolol: <0.1%
  
  Metoprolol: 0.1%
  
  Chlorpyrifos: 0.3%
  
  Naproxen: 2.2%
  
  Clofibrinic acid: 0.3%
  
  Nonylphenol: 1.3%
  
  Diclofenac: <0.1%
  
  Octylphenol: 0.6%
  
  Erythromycine: 0.1%
  
  Propanolol: 0.1%
  
  Fenitrothion: 0.5%
  
  Vinclozolin: <0.1%
  
  Fenofibrate: 0.5%
  
  Zearalenon: 0.2%
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:300
  • Immunoaffinity Purification
• Application Notes: The antibody may be used in ELISA and Immuno-affinity-chromatography (IAC). Suggested dilution 1:300 in ELISA (this titer is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance when tested with ELISA). Plates are coated with 400 ng/ml ovalbumin-conjugated derivative. HRP-conjugated anti-rabbit IgG should be used as a tracer in a 1:8,000 dilution.
  
  Immuno-affinity-chromatography.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antiserum
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Ibuprofen Antibody

• 2-(4-ISOBUTYLPHENYL)PROPIONIC ACID
• 4-Isobutylhydratropic acid

Background

Ibuprofen is an anti-inflammatory drug used for pain relief, fever reduction and against swelling. It is one of the most sold over-the-counter drugs. It is found in the environment, especially in the water environment in the vicinity of large cities and hospitals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.