I-Ab Antibody (AF6-120.1) [FITC]

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Flow Cytometry: I-Ab Antibody (AF6-120.1) [FITC] [NB100-77616] - C57BL/6 mouse splenocytes stained with AF6-120.1 FITC

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-77616
    • Availability
      Product Discontinued

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I-Ab Antibody (AF6-120.1) [FITC] Summary

Immunogen
C57BL/10J splenocytes
Specificity
I-Ab (AF6-120.1)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
H2-Ab1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one millioncells in 100 microliter volume. Immunohistochemistry-frozen sections. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Mouse b haplotype, Cross-reacts with H-2 k and H-2 u haplotypes. Does not react with other haplotypes (e.g., d, f, q, r, s).. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Purity
Protein A or G purified

Alternate Names for I-Ab Antibody (AF6-120.1) [FITC]

  • MHC class II Ab

Background

The AF6-120.1 antibody reacts with the I-Ab MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The AF6-120.1 antibody cross-reacts with H-2k and H-2u haplotypes; this antibody does not cross-react with other haplotypes (d, f, q, r, s).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Calculators

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Bioinformatics

Gene Symbol H2-Ab1