Recombinant Human Hydroxyacid Oxidase-1/HAO-1 Protein

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SDS-Page: Hydroxyacid Oxidase-1/HAO-1 Recombinant Protein [NBP1-72412]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human Hydroxyacid Oxidase-1/HAO-1 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-370 of Human Hydroxyacid Oxidase-1/HAO-1

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMLPR LICINDYEQH AKSVLPKSIY DYYRSGANDE ETLADNIAAF SRWKLYPRML RNVAETDLST SVLGQRVSMP ICVGATAMQR MAHVDGELAT VRACQSLGTG MMLSSWATSS IEEVAEAGPE ALRWLQLYIY KDREVTKKLV RQAEKMGYKA IFVTVDTPYL GNRLDDVRNR FKLPPQLRMK NFETSTLSFS PEENFGDDSG LAAYVAKAID PSISWEDIKW LRRLTSLPIV AKGILRGDDA REAVKHGLNG ILVSNHGARQ LDGVPATIDV LPEIVEAVEG KVEVFLDGGV RKGTDVLKAL ALGAKAVFVG RPIVWGLAFQ GEKGVQDVLE ILKEEFRLAM ALSGCQNVKV IDKTLVRKNP LAVSKI

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
HAO1
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.5M NaCl
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human Hydroxyacid Oxidase-1/HAO-1 Protein

  • (S)-2-hydroxy-acid oxidase
  • EC 1.1.3.15
  • Glycolate oxidase
  • GOX
  • GOX1MGC142227
  • GOXMGC142225
  • HAO1
  • HAO-1
  • HAOX1
  • hydroxyacid oxidase (glycolate oxidase) 1
  • hydroxyacid oxidase 1
  • Hydroxyacid Oxidase1
  • Hydroxyacid Oxidase-1

Background

HAO1, also known as glycolate oxidase, is a member of the superfamily of the alpha hydroxy acid oxidases(HAO) enzymes. It catalyzes the FMN mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide. It is most highly expressed in liver and pancreas and is most active on twocarbon substrates such as glycolate. Recently, it has been identified as a major contributor to hyperoxaluria, a disorder in which large deposits of calcium oxalate form kidney stones. Recombinant human HAO1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for Hydroxyacid Oxidase-1/HAO-1 Recombinant Protein (NBP1-72412) (0)

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FAQs for Hydroxyacid Oxidase-1/HAO-1 Recombinant Protein (NBP1-72412). (Showing 1 - 1 of 1 FAQ).

  1. I will be using this protein for enzyme assay. Do you have a protocol so that I can determine if I need other reagents.
    • For our protein, NBP1-72412, we have only tested this protein as a positive control in SDS-PAGE gels. Therefore, we do not have a protocol for enzyme assay. I am sorry for any inconvenience. While we do not have an enzyme assay protocol available, I was able to find the following paper that may help to determine what reagents you may need for this application: science direct site.

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Bioinformatics

Gene Symbol HAO1
Entrez