HVEM/TNFRSF14 Antibody (122)

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Flow Cytometry: HVEM/TNFRSF14 Antibody (122) [NB100-78025] - Human peripheral blood lymphocytes stained with purified 122, followed by biotinylated anti-mouse IgG and Sav-PE.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-78025
    • Availability
      Product Discontinued

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HVEM/TNFRSF14 Antibody (122) Summary

Immunogen
recombinant humanHVEM protein
Specificity
HVEM (122)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TNFRSF14
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume or 100 microliter of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Positive Control
HVEM/TNFRSF14 Lysate (NBL1-17150)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for HVEM/TNFRSF14 Antibody (122)

  • ATAR
  • CD270 antigen
  • CD270
  • CD40-like protein
  • Herpes virus entry mediator A
  • Herpesvirus entry mediator A
  • HveA
  • HVEM
  • HVEMTR2HVEAATAR
  • LIGHTR
  • TNFRSF14
  • tumor necrosis factor receptor superfamily member 14
  • tumor necrosis factor receptor superfamily, member 14 (herpesvirus entrymediator)
  • Tumor necrosis factor receptor-like 2
  • tumor necrosis factor receptor-like gene2

Background

The 122 antibody recognizes human HVEM also known as herpesvirus entry mediator A, tumor necrosis factor receptor superfamily, member 14, TNFRSF14, and tumor necrosis factor receptor like 2. HVEM, a member of the TNFR superfamily, is a type I transmembrane protein containing 2 TNF receptor domains with a predicted molecular weight of approximately 30 kD. HVEM is widely expressed in blood vessels, brain, heart, kidney, liver, lung, prostate, spleen, thymus and other organs. Resting T cells and na iumlve and memory B cells express high levels of HVEM as well.In humans, HVEM is not expressed in germinal center B cells. Immature dendritic cells express high levels of HVEM that is downregulated upon maturation. HVEM plays an important role in herpes simplex virus pathogenesis by enhancing entry into cells. Signaling through HVEM activates JNK1, NF- kappaB and AP-1 to control gene expression in response to infection or cellular stress and activate the immune response. HVEM binds to LIGHT and has also been shown to associate with several other proteins including TRAF1, TRAF2, TRAF3, TRAF5, B and T lymphocyte associated protein (BTLA), and estrogen receptor alpha. The 122 antibody has been shown to be useful for flow cytometry, Western blot, and ELISA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional HVEM/TNFRSF14 Products

Bioinformatics Tool for HVEM/TNFRSF14 Antibody (NB100-78025)

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Diseases for HVEM/TNFRSF14 Antibody (NB100-78025)

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PTMs for HVEM/TNFRSF14 Antibody (NB100-78025)

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Bioinformatics

Gene Symbol TNFRSF14
Entrez