Huntingtin Antibody

Product Discontinued

Huntingtin Antibody Summary

• Immunogen: A synthetic peptide from internal part of rat Huntingtin (Huntington disease protein homolog, Htt, HD protein homolog) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 87% identity with mouse and human sequences.
• Localization: subcellular location: cytoplasm. nucleus. tissue specificity: expressed to a high degree in all the regions of the brain of adults and in meiotic cells of the testis.
• Specificity: Huntingtin.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HTT
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:300-1:1000

Reactivity Notes

Rat and Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute with 0.1 ml sterilized water to desired concentration.

Alternate Names for Huntingtin Antibody

• HD protein
• HD
• HTT
• huntingtin (Huntington disease)
• Huntingtin
• IT15
• IT15HDHuntington disease protein

Background

Huntington's disease (HD) is a neurodegenerative disorder caused by an expanding polyglutamine repeat in the huntingtin gene. HD is a mid-life onset autosomal dominant neurodegeneative disease that is characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10-20 years. The HD gene product is widely expressed in human tissues, with the highest level of expression in the brain. The huntingtin gene product is expressed at similar levels in patients and controls, which suggests that the expansion of the polyglutamine repeat induces a toxic gain of function perhaps through interactions with other cellular proteins. Using yeast two-hybrid system, HAP1 (huntingtin associated protein 1) has been identified, that associates with huntingtin protein. The In vitro data suggest that the association between HAP1 and huntingtin is enhanced by increasing length of glutamine repeat.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HTT
• Entrez:
  • 15194(Mouse)
  • 29424(Rat)
• Uniprot:
  • P51111(Rat)