Huntingtin Antibody (HDC8A4)

Images

 
Western Blot: Huntingtin Antibody (HDC8A4) [NB600-1198] - Western Blot: Total protein extract of normal human cerebral cortex separated as a strip on a 3-12.5% gradient SDS-PAGE gel and Western blotted. The blot was ...read more

Product Details

Summary
Reactivity Hu, Mu, RbSpecies Glossary
Applications WB, IHC, IHC-Fr, IP
Clone
HDC8A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Huntingtin Antibody (HDC8A4) Summary

Immunogen
Recombinant fragment, corresponding to amino acids 2703-2911 of Huntingtin.
Localization
Cytoplasmic
Specificity
This reacts with an epitope corresponding to the HDC region (2703 - 2911 amino acids) of the huntingtin protein
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HTT
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
Immunohistology - frozen (paraffin not tested)Western blotting - detects a 350kD band on western blots but also detects smaller degradation products of huntingtin. This clone recognizes both denatured and native huntingtin in human brain.
Positive Control
Brain Lysate (NB820-59177)

Reactivity Notes

Cross-reacts with Human, Rabbit and Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Huntingtin Antibody (HDC8A4)

  • HD protein
  • huntingtin (Huntington disease)
  • huntingtin
  • IT15HDHuntington disease protein

Background

The combined use of clones HDC8A4, HDB4E10 and HDA3E10 demonstrate that huntingtin is enriched in neuronal cells in the brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-35213
Species: Hu
NB100-1269
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA
44110002
Species: Hu
Applications: WB, ELISA, ICC/IF
248-BD/CF
Species: Hu, Mu, Rt, Ca, Eq
AF5739
Species: Hu, Mu, Rt
Applications: WB
NBP1-86713
Species: Hu
Applications: IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NB500-418
Species: Hu, Po, Rb
Applications: WB, B/N, ELISA, ICC/IF, IHC, IHC-P, RIA
NB100-56708
Species: Hu, Mu, Rt, Po, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
NBP1-35703
Species: Hu, Mu
Applications: WB, IHC, IHC-P
9134-TN
Species: Hu

Publications for Huntingtin Antibody (NB600-1198) (0)

There are no publications for Huntingtin Antibody (NB600-1198).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Huntingtin Antibody (NB600-1198) (0)

There are no reviews for Huntingtin Antibody (NB600-1198). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Huntingtin Antibody (NB600-1198) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Huntingtin Products

Bioinformatics Tool for Huntingtin Antibody (NB600-1198)

Discover related pathways, diseases and genes to Huntingtin Antibody (NB600-1198). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Huntingtin Antibody (NB600-1198)

Discover more about diseases related to Huntingtin Antibody (NB600-1198).
 

Pathways for Huntingtin Antibody (NB600-1198)

View related products by pathway.

PTMs for Huntingtin Antibody (NB600-1198)

Learn more about PTMs related to Huntingtin Antibody (NB600-1198).
 

Research Areas for Huntingtin Antibody (NB600-1198)

Find related products by research area.

Blogs on Huntingtin

There are no specific blogs for Huntingtin, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Huntingtin Antibody (HDC8A4) and receive a gift card or discount.

Bioinformatics

Gene Symbol HTT
Entrez
Uniprot