Huntingtin Antibody (1A771)

Product Discontinued

Huntingtin Antibody (1A771) Summary

• Immunogen: An Htt mutant protein containing 62 glutamine amino acid repeats was used as immunogen.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: HTT
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Frozen 2 ug/ml
  • Western Blot 1 ug/ml
• Application Notes: Clone 1A771 binds to the expanded polyQ repeat form of Htt in WB, showing no detectable binding to normal Htt in WB.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Huntingtin Antibody (1A771)

• HD protein
• HD
• HTT
• huntingtin (Huntington disease)
• Huntingtin
• IT15
• IT15HDHuntington disease protein

Background

Huntington's disease (HD) is a neurodegenerative disorder caused by an expanding polyglutamine repeat in the huntingtin (Htt) gene. HD is a mid-life onset autosomal dominant neurodegenerative disease that is characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10-20 years. The HD gene product HTT is widely expressed in human tissues, with the highest level of expression in the brain. Furthermore, HTT is expressed at similar levels in HD patients and controls, which suggests that the expansion of the polyglutamine repeat induces a toxic gain of function perhaps through interactions with other cellular proteins. Using yeast two-hybrid system Li et al., have identified a protein, HAP1 (huntington associated protein 1), that associates with HTT. The in vitro data suggest that the association between HAP1 and HTT is enhanced by increasing length of the glutamine repeat in HTT.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HTT
• Entrez:
  • 3064(Human)
  • 15194(Mouse)
• Uniprot:
  • P42858(Human)