Huntingtin Antibody (1A771)

Images

 
Western Blot: Huntingtin Antibody (1A771) [NB100-56703] - Analysis of Htt in four different lymphoblast HD cell lines extracts each expressing ~65 (mutant) and ~20 (normal) CAG repeats. Lanes 1-4. A, HTT mAb clone ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-Fr
Clone
1A771
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Huntingtin Antibody (1A771) Summary

Immunogen
An Htt mutant protein containing 62 glutamine amino acid repeats was used as immunogen.
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
HTT
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Frozen 2 ug/ml
Application Notes
Clone 1A771 binds to the expanded polyQ repeat form of Htt in WB, showing no detectable binding to normal Htt in WB.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Huntingtin Antibody (1A771)

  • HD protein
  • huntingtin (Huntington disease)
  • huntingtin
  • IT15HDHuntington disease protein

Background

Huntington's disease (HD) is a neurodegenerative disorder caused by an expanding polyglutamine repeat in the huntingtin (Htt) gene. HD is a mid-life onset autosomal dominant neurodegenerative disease that is characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10-20 years. The HD gene product HTT is widely expressed in human tissues, with the highest level of expression in the brain. Furthermore, HTT is expressed at similar levels in HD patients and controls, which suggests that the expansion of the polyglutamine repeat induces a toxic gain of function perhaps through interactions with other cellular proteins. Using yeast two-hybrid system Li et al., have identified a protein, HAP1 (huntington associated protein 1), that associates with HTT. The in vitro data suggest that the association between HAP1 and HTT is enhanced by increasing length of the glutamine repeat in HTT.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56703AF405
Alexa Fluor 488 Labeled NB100-56703AF488
Alexa Fluor 647 Labeled NB100-56703AF647
Alexa Fluor 700 Labeled NB100-56703AF700
Biotin Labeled NB100-56703B
DyLight 350 Labeled NB100-56703UV
DyLight 405 Labeled NB100-56703V
DyLight 488 Labeled NB100-56703G
DyLight 550 Labeled NB100-56703R
DyLight 650 Labeled NB100-56703C
DyLight 680 Labeled NB100-56703FR
DyLight 755 Labeled NB100-56703IR
HRP Labeled NB100-56703H

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Bioinformatics

Gene Symbol HTT
Entrez
Uniprot