Human Total 25-OH Vitamin D IVD ELISA Kit


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Reactivity HuSpecies Glossary
Applications ELISA

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Human Total 25-OH Vitamin D IVD ELISA Kit Summary

Additional Information
For In Vitro Diagnostic Use. Available only in the USA.
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
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Sample Volume
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Read Publications using RDKAP1971.

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Total 25-OH Vitamin D IVD ELISA Kit

  • 25OH Vitamin D
  • 25-OH Vitamin D


25-hydroxy-vitamin D3 (25(OH)D3) is synthesized in the liver and is the primary circulating form of vitamin D. Its blood concentration, which reflects 25(OH)D3 produced by exposure to ultraviolet B, as well as dietary and vitamin D supplementation, is felt to be the best indicator of vitamin D status. 25(OH)D3 is metabolized to 1-alpha,25(OH)2D3 in the proximal tubular cells of the kidney by the enzyme 1-alpha-hydroxylase. The vitamin D structure is similar to that of classic steroid hormones, such as estradiol, cortisol, and aldosterone in that they have the same root cyclopentanoperhydrophenanthrene ring structure. It has been shown that the active steroid hormone 1-alpha,25(OH)2D3 is essential for life in higher animals. Besides playing important roles in calcium homeostasis and bone mineral metabolism, it is now known to play a role in cellular differentiation, inhibition of cell growth, immune regulation and the prevention of neoplastic transformation. The active form of vitamin D3, 1-alpha,25(OH)2D3, acts both through its cellular receptor, the vitamin D receptor (VDR), and through other extrarenal targets in an autocrine and paracrine manner where 1-alpha-hydroxylase is present.

Publications for 25-OH Vitamin D (RDKAP1971)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

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Showing Publications 1 - 7 of 7.
Publications using RDKAP1971 Applications Species
MF Schleu, B Barreto-Du, MB Arriaga, M Araujo-Per, AM Ladeia, BB Andrade, ML Lima Lower Levels of Vitamin D Are Associated with an Increase in Insulin Resistance in Obese Brazilian Women Nutrients, 2021;13(9):. 2021 [PMID: 34578857] (Human) Human
T Barker, HT May, JR Doty, DL Lappe, KU Knowlton, J Carlquist, K Konery, S Inglet, B Chisum, O Galenko, JL Anderson, JB Muhlestein Vitamin D supplementation protects against reductions in plasma 25-hydroxyvitamin D induced by open-heart surgery: Assess-d trial Physiological Reports, 2021;9(3):e14747. 2021 [PMID: 33580636] (Human) Human
Y Jiang, W Luo, B Wang, Z Yi, P Gong, Y Xiong 1&alpha,25-Dihydroxyvitamin D3 ameliorates diabetes-induced bone loss by attenuating FoxO1-mediated autophagy The Journal of Biological Chemistry, 2021;0(0):100287. 2021 [PMID: 33450223] (Mouse) Mouse
A Alrafiah, E Al-Ofi, MT Obaid, N Alsomali Assessment of the Levels of Level of Biomarkers of Bone Matrix Glycoproteins and Inflammatory Cytokines from Saudi Parkinson Patients Biomed Res Int, 2019;2019(0):2690205. 2019 [PMID: 31205938] (Human) Human
Q Kang, X Zhang, S Liu, F Huang Correlation between the vitamin D levels and asthma attacks in children: Evaluation of the effects of combination therapy of atomization inhalation of budesonide, albuterol and vitamin D supplementation on asthmatic patients Exp Ther Med, 2018;15(1):727-732. 2018 [PMID: 29399078] (Human) Human
Y Xiong, Y Zhang, N Xin, Y Yuan, Q Zhang, P Gong, Y Wu 1?,25-Dihydroxyvitamin D3 promotes bone formation by promoting nuclear exclusion of the FoxO1 transcription factor in diabetic mice J. Biol. Chem., 2017;0(0):. 2017 [PMID: 29042442] (Mouse) Mouse
AB Abdel-Naim, AA Alghamdi, MM Algandaby, FA Al-Abbasi, AM Al-Abd, HM Abdallah, AM El-Halawan, M Hattori Phenolics Isolated from Aframomum meleguta Enhance Proliferation and Ossification Markers in Bone Cells Molecules, 2017;22(9):. 2017 [PMID: 28869564] (Human) Human

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