Human IgM, Fc5u Native Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IP
Concentration
1.0 mg/ml

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Human IgM, Fc5u Native Protein Summary

Description
A full-length protein corresponding to Human IgM, Fc5u.

Source: Human Serum

Preparation
Method
Human IgM (myeloma) Fc5u fragment has been prepared from Human IgM myeloma protein by digestion with trypsin followed by column chromatography. Purity was assessed by SDS-PAGE and HPLC to be greater than 95%. A single precipitin arc was observed against anti-human IgM Fc5u and anti-human serum when assayed by immuno-electrophoresis at a concentration of 20 mg/ml. No reaction was observed against anti-Trypsin, anti-human IgG F(ab')2, anti-human IgG F(c), anti-human Kappa or anti-human Lambda.
Protein/Peptide Type
Native Protein
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ion exchange chromatography

Background

Human IgM (myeloma) FC5U fragment consists of only the u (mu) chain of the Fc fragment. Immunoglobulin M is the largest antibody isotype and the first to be secrected against an initial exposure to antigen. IgM is predominantly produced in the spleen. IgM is formed from covalently linking 5 immunoglobulins together. Due to this large size, IgM is typically isolated to the serum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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