Human IgG, Fc Native Protein


SDS-Page: Human IgG, Fc Native Protein [NBP1-96973] - SDS-PAGE results of Human IgG F(c) Fragment. Lane 1: reduced Human IgG F(c) Fragment. Lane 2: Opal Prestained Molecular Weight Ladder. Lane 3: non-reduced Human IgG more
SDS-Page: Human IgG, Fc Native Protein [NBP1-96973] - SDS-PAGE of Human IgG Fc. Lane 1: Non-reduced Human IgG Fc Lane 2: Non-reduced Human IgG Whole Molecule Lane 3: Non-reduced Human IgG Fab Fragment Lane 4: more

Product Details

Reactivity HuSpecies Glossary
Applications WB, ELISA, PAGE
1.0 mg/ml

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Human IgG, Fc Native Protein Summary

Native Human IgG, Fc protein.

Source: Human Serum

HUMAN IgG F(c) was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti-Human IgG and anti-Human IgG F(c). No reaction was observed against anti-Human IgG F(ab')2 or anti-Papain.
Protein/Peptide Type
Native Protein
Immunoelectrophoresis (IEP)


  • Western Blot 1:1000
  • ELISA 1:5000-1:50000

Packaging, Storage & Formulations

Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% Sodium Azide
1.0 mg/ml
Immunoelectrophoresis (IEP)


Human IgG Fc purified protein is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of Human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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