Human IgG, Fc Native Protein

Images

 
SDS-Page: Human IgG, Fc Native Protein [NBP1-96973] - SDS-PAGE results of Human IgG F(c) Fragment. Lane 1: reduced Human IgG F(c) Fragment. Lane 2: Opal Prestained Molecular Weight Ladder. Lane 3: non-reduced Human IgG ...read more
SDS-Page: Human IgG, Fc Native Protein [NBP1-96973] - SDS-PAGE of Human IgG Fc. Lane 1: Non-reduced Human IgG Fc Lane 2: Non-reduced Human IgG Whole Molecule Lane 3: Non-reduced Human IgG Fab Fragment Lane 4: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, PAGE
Concentration
1.0 mg/ml

Order Details

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Human IgG, Fc Native Protein Summary

Description
Native Human IgG, Fc protein.

Source: Human Serum

Preparation
Method
HUMAN IgG F(c) was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti-Human IgG and anti-Human IgG F(c). No reaction was observed against anti-Human IgG F(ab')2 or anti-Papain.
Protein/Peptide Type
Native Protein
Purity
Immunoelectrophoresis (IEP)

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:5000-1:50000

Packaging, Storage & Formulations

Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunoelectrophoresis (IEP)

Background

Human IgG Fc purified protein is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of Human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Video Protocols

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