Human IgG, Fab2 Native Protein

Images

 
SDS-Page: Human IgG, Fab2 Full length Protein [NBP1-96779] - Lane 1: Human F(ab')2 - Non-Reduced. Lane 2: Human F(ab')2 - Reduced. Load: 1.0 ug per lane.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IP, PAGE
Concentration
LYOPH

Order Details

Human IgG, Fab2 Native Protein Summary

Description
A full-length protein corresponding to Human IgG, Fab2.

Source: Human Serum

Preparation
Method
Human IgG F(ab')2 was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti-Human IgG and anti-Human IgG F(ab')2. No reaction was observed against anti-Human IgG F(c) or anti-Pepsin.
Protein/Peptide Type
Native Protein
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:5000-1:50000
  • Immunoprecipitation 1:10-1:500
  • SDS-Page 1:100-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 1.0 ml deionized water.

Notes

This product is stable for several weeks at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use.

Background

Human IgG F(ab')2 purified protein is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. Human IgG F(ab')2 molecules lack the Fc portion of Human IgG and therefore receptors that bind Human IgG Fc will not bind Human IgG F(ab')2 molecules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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