Human IgG, Fab Native Protein

Images

 
SDS-Page: Human IgG, Fab Native Protein [NBP1-97020] - SDS-PAGE results of Human IgG Fab Fragment. Lane 1: reduced Human IgG Fab Fragment (5ug). Lane 2: reduced Human IgG Fab Fragment (1ug). Lane 3: Opal Prestained ...read more
SDS-Page: Human IgG, Fab Native Protein [NBP1-97020] - SDS-Page of Human Fab. Lane 1: Human Fab - Non-Reduced. Lane 2: Human Fab - Reduced. Load: 1.0 ug per lane. Predicted/Observed size - Non-Reduced: 50 kDa, 50 kDa ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, PAGE
Concentration
2.0 mg/ml

Human IgG, Fab Native Protein Summary

Description
Native Human IgG, Fab protein.

Source: Human Serum

Preparation
Method
Human IgG Fab fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Human IgG Fab fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti- Human IgG and anti- Human IgG F(ab')2. No reaction was observed against anti- Human IgG F(c) or anti-Papain.
Protein/Peptide Type
Native Protein
Purity
Immunoelectrophoresis (IEP)

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10 - 1:500
  • SDS-Page 1:100 - 1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
2.0 mg/ml
Purity
Immunoelectrophoresis (IEP)

Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The F(ab) fragment is the portion of the antibody that binds to the antigen target. The immunoglobulin Fab also possesses one constant and one variable region of both the heavy and light chain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Human IgG, Fab Native Protein (NBP1-97020). (Showing 1 - 2 of 2 FAQ).

  1. Can I have the sequence of this protein?
    • The polyclonal IgG was purified from human serum and is not sequenced.
  2. Would you please help confirm if this protein contains any thiol group (-SH)?
    • The molecules are heterogenous and most likely have thiol groups.

Additional Human IgG, Fab Products

Human IgG, Fab NBP1-97020

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