Recombinant Human Human Growth Hormone Protein (NBC1-18450)

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SDS-Page: Human Growth Hormone Protein [NBC1-18450] - Growth hormone, 22.2 kDa (192 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human Human Growth Hormone Protein Summary

Description
An un-tagged recombinant protein corresponding to the amino acids 27-217 of Human Growth Hormone

Source: E.coli

Amino Acid Sequence: MFPTIPLSRL FDNAMLRAHR LHQLAFDTYQ EFEEAYIPKE QKYSFLQNPQ TSLCFSESIP TPSNREETQQ KSNLELLRIS LLLIQSWLEP VQFLRSVFAN SLVYGASDSN VYDLLKDLEE GIQTLMGRLE DGSPRTGQIF KQTYSKFDTN SHNDDALLKN YGLLYCFRKD MDKVETFLRI VQCRSVEGSC GF

Preparation
Method
E.coli
Details of Functionality
Measured in a cell proliferation assay using Nb2 Rat lymphoma cells. The ED50 for this effect is < 0.5 ng/ml.
Protein/Peptide Type
Biologically Active Protein
Gene
GH1
Purity
>95% pure by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Theoretical MW
22.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate-buffered saline (pH 7.4), 88 mM mannitol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human Human Growth Hormone Protein

  • GHIGHD1B
  • GHNGrowth hormone
  • GH-Nsomatotropin
  • growth hormone 1Pituitary growth hormone
  • hGH-N

Background

Human growth hormone(hGH, somatotropin), a protein hormone from the anterior lobe of the pituitary gland, comprises a single polypeptide chain of 191 amino acid residues. The growth hormone amino acid sequence varies considerably between species, and non-primate growth hormones have little activity in man. Its main function is the stimulation of somatic and bone growth, as well as an increase in the size and mass of organs and tissues. Recombinant human growth hormone(rhGH ) was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol GH1