Human Growth Hormone Overexpression Lysate (Native)

Images

 
Western Blot: Human Growth Hormone Overexpression Lysate (Adult Normal) [NBL1-11063] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Human Growth Hormone.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Human Growth Hormone Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC209608 and based on accession number NM_022559. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens growth hormone 1 (GH1), transcript variant 2, mRNA.
Gene
GH1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
22.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Human Growth Hormone

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Human Growth Hormone Overexpression Lysate (Native)

  • GHIGHD1B
  • GHNGrowth hormone
  • GH-Nsomatotropin
  • growth hormone 1Pituitary growth hormone
  • hGH-N

Background

The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. [provided by RefSeq]. Transcript Variant: This variant (2) utilizes an alternative splice acceptor site 45 nt into exon 3 to generate the 20-kDa isoform (2) which has an internal deletion relative to the predominant 22-kDa isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF682
Species: Hu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
NBP2-29915
Species: Hu
Applications: WB, ICC/IF, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
H00005087-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
MAB2358
Species: Hu, Mu
Applications: IHC, ICC
NB100-1533
Species: Hu, Mu, Po, Sh
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
NBP2-34014
Species: Hu
Applications: IHC, IHC-P
NBP2-38770
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-30475
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP, PLA
AF1360
Species: Mu
Applications: WB, IHC
MAB8200
Species: Hu, Mu
Applications: IHC
MAB1249
Species: Hu, Mu
Applications: CyTOF-ready, ICC, ICFlow
NBP2-56762
Species: Hu
Applications: WB, ICC/IF
NBP1-92273
Species: Hu
Applications: IHC, IHC-P
NBL1-11063
Species: Hu
Applications: WB

Publications for Human Growth Hormone Lysate (NBL1-11063) (0)

There are no publications for Human Growth Hormone Lysate (NBL1-11063).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Human Growth Hormone Lysate (NBL1-11063) (0)

There are no reviews for Human Growth Hormone Lysate (NBL1-11063). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Human Growth Hormone Lysate (NBL1-11063) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Human Growth Hormone Products

Bioinformatics Tool for Human Growth Hormone Lysate (NBL1-11063)

Discover related pathways, diseases and genes to Human Growth Hormone Lysate (NBL1-11063). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Human Growth Hormone Lysate (NBL1-11063)

Discover more about diseases related to Human Growth Hormone Lysate (NBL1-11063).
 

Pathways for Human Growth Hormone Lysate (NBL1-11063)

View related products by pathway.

PTMs for Human Growth Hormone Lysate (NBL1-11063)

Learn more about PTMs related to Human Growth Hormone Lysate (NBL1-11063).

Blogs on Human Growth Hormone

There are no specific blogs for Human Growth Hormone, but you can read our latest blog posts.

Customers Who Bought This Also Bought

IGF-II
292-G2-050
POMC Antibody
NB100-1533

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Human Growth Hormone Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol GH1