HUCE1 Antibody

Product Discontinued

HUCE1 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 291-340 of Human Cerebral 1.
• Specificity: Cerebral 1 (L310) antibody detects endogenous levels of Cerebral 1 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RRP8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of Cerebral 1 (L310) pAb in extracts from MCF-7 cells. Immunohistochemistry (IHC) analyzes of Cerebral 1 (L310) pAb in paraffin-embedded human breast carcinoma tissue.
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HUCE1 Antibody

• Cerebral protein 1
• KIAA0409EC 2.1.1.-
• NML
• nucleomethylin
• ribosomal RNA processing 8, methyltransferase, homolog (yeast)
• ribosomal RNA-processing protein 8
• RRP8 methyltransferase homolog

Background

Cerebral 1 also called hucep-1, KIAA0409 or RRP8, is an essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RRP8
• Entrez:
  • 23378(Human)