HSPB8/HSP22 Antibody (1E3)

Images

 
Western Blot: Hsp22 Antibody (1E3) [NBP2-02576] Analysis of extracts (35ug) from 9 different cell lines by using anti-Hsp22 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Hsp22 Antibody (1E3) [NBP2-02576] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hsp22.
Flow Cytometry: Hsp22 Antibody (1E3) [NBP2-02576] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Hsp22 antibody, and then analyzed ...read more
Western Blot: Hsp22 Antibody (1E3) [NBP2-02576] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Hsp22 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Flow Cytometry: Hsp22 Antibody (1E3) [NBP2-02576] - Analysis of Jurkat cells, using anti-Hsp22 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Rt, MkSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
1E3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HSPB8/HSP22 Antibody (1E3) Summary

Immunogen
Full length human recombinant protein of human HSPB8(NP_055180) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HSPB8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HSPB8/HSP22 Antibody (1E3)

  • Alpha-crystallin C chain
  • CMT2L
  • CRYAC
  • DHMN2
  • E2IG1
  • E2IG1E2-induced gene 1 protein
  • H11
  • H11DHMN2
  • heat shock 22kDa protein 8
  • heat shock 27kDa protein 8
  • heat shock protein beta-8
  • HMN2
  • HMN2A
  • HSP22
  • HSP22CMT2L
  • HSPB8
  • Protein kinase H11
  • Small stress protein-like protein HSP22

Background

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol HSPB8
Entrez