HSP90AB1 Antibody (H90-10)

Images

 
Western Blot: gp96/HSP90B1/GRP94 Antibody (H90-10) [NB110-61640] - analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Primary Antibody: Mouse Anti-Hsp90 ...read more
Immunocytochemistry/ Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (H90-10) [NB110-61640] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 ...read more
Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (H90-10) [NB110-61640] - Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 ...read more
Flow Cytometry: gp96/HSP90B1/GRP94 Antibody (H90-10) [NB110-61640] - Analysis of the APC conjugate of this antibody. An intracellular stain was performed on Jurkat cells with gp96/HSP90B1/GRP94 Antibodyantibody ...read more
Western Blot: gp96/HSP90B1/GRP94 Antibody (H90-10) [NB110-61640] - analysis of Human cell lysates from various cell lines showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Load: ...read more
Western Blot: gp96/HSP90B1/GRP94 Antibody (H90-10) [NB110-61640] - analysis of Human HeLa cell lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Primary Antibody: Mouse ...read more
Immunocytochemistry/ Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (H90-10) [NB110-61640] - analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. ...read more
Immunocytochemistry/ Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (H90-10) [NB110-61640] - Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at ...read more
Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (H90-10) [NB110-61640] - Cancerous human colon tissue.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
Clone
H90-10
Clonality
Monoclonal
Host
Mouse
Concentration
1 mg/ml

HSP90AB1 Antibody (H90-10) Summary

Immunogen
Recombinant human HSP90beta.
Localization
Cytoplasm, Melanosome
Marker
ER Stress Marker
Specificity
Detects 90kDa. Detects HSP90 beta in all reactive species except in Chicken, where it detects both alpha and beta isoforms.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HSP90AB1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
1 ug/ml of HSP90 Antibody was sufficient for detection of HSP90beta in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Reactivity Notes

Based on homology, It's predicted to detect Mouse, Rat, Rabbit, Chicken, Canine, Fish, White Sucker Fish (Catostomus commersonii)SharkGummy Shark (Mustelus antarcticus) and School Shark (Galeorhinus galeus).

Alternate Names for HSP90AB1 Antibody (H90-10)

  • D6S182
  • Heat shock 84 kDa
  • heat shock 90kD protein 1, beta
  • heat shock 90kDa protein 1, beta
  • Heat Shock Protein 90 Alpha Family Class B Member 1
  • Heat Shock Protein 90 KDa
  • heat shock protein 90kDa alpha (cytosolic), class B member 1
  • Heat Shock Protein 90kDa Alpha Family Class B Member 1
  • heat shock protein HSP 90-beta
  • HSP 84
  • HSP 90
  • HSP84
  • HSP90B
  • HSP90-BETA
  • HSPC2
  • HSPCB

Background

HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms alpha and beta, which share 85% sequence amino acid homology. The two isoforms of Hsp90, are expressed in the cytosolic compartment (1). Despite the similarities, HSP90alpha exists predominantly as a homodimer while HSP90beta; exists mainly as a monomer.(2) From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex. (3-6) Furthermore, Hsp90 is highly conserved between species; having 60% and 78% amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively. Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite it's label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1'2% ofcytosolic protein). It carries out a number of housekeeping functions including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90- regulated proteins that have been discovered to date are involved in cell signaling. (7-8). The number of proteins now know to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5 When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function (9).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB120-2928
Species: Hu, Mu, Rt, Pm, Rb, Sh
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-81696
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-02459
Species: Hu, Mu, Rt, Ch, Dr, Pm
Applications: WB, ELISA, IHC, IHC-P
NBP1-97868
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, GP, Ha, Mk, Rb, Sh
Applications: WB, EM, EIA, Flow, ICC/IF, IHC, IHC-P, IP
NB300-619
Species: Hu, Mu, Rt, Bv, Ch, Ha
Applications: WB, ICC/IF, IHC, IHC-P, IP
MAB23141
Species: Hu
Applications: WB, ICC
NBP1-77397
Species: Hu, Mu, Rt, Ha, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
NB120-2788
Species: Hu, Mu, Rt, Bv, Fe, Fi, Ha, Pm
Applications: WB, B/N, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-97475
Species: Hu, Mu, Rt, Ge, Pm, Rb
Applications: WB, ELISA, EIA, GS, IP
NBP1-06274
Species: Hu, Mu, Rt, Ch, Dr, Sh
Applications: WB, Simple Western, Flow, ICC/IF
NB300-731
Species: Hu, Mu, Rt, GP, Rb, Sh, Ye, Am(-), Av(-), Pm(-)
Applications: WB, ChIP, B/N, Flow, GS, ICC/IF, IHC, IHC-P, IP
NB100-91818
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB600-501
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, GP, Ha, Le, Ma, Pm, Rb, Sh, Sq, Ze, Dr(-)
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB100-64792
Species: Hu, Mu, Rt, Rb
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr
NBP1-89662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
MAB1489
Species: Hu
Applications: WB
NB300-221
Species: Hu, Mu, Rt, Po, Av, Bv, Ch, Eq, Ha, Op, Pm, Sh, Ze
Applications: WB, Simple Western, ICC/IF, IHC, IP, ChIP
NB600-101
Species: Hu, Mu, Rt, Bv, Ha, Pm
Applications: WB, Simple Western, B/N, DB, EM, ICC/IF, IHC, IHC-P, IP, PA

Publications for HSP90AB1 Antibody (NB110-61640) (0)

There are no publications for HSP90AB1 Antibody (NB110-61640).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HSP90AB1 Antibody (NB110-61640) (0)

There are no reviews for HSP90AB1 Antibody (NB110-61640). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSP90AB1 Antibody (NB110-61640) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-61640AF405
Alexa Fluor 488 Labeled NB110-61640AF488
Alexa Fluor 647 Labeled NB110-61640AF647
Alexa Fluor 700 Labeled NB110-61640AF700
Allophycocyanin Labeled NB110-61640APC
DyLight 350 Labeled NB110-61640UV
DyLight 405 Labeled NB110-61640V
DyLight 405LS Labeled NB110-61640V3
DyLight 488 Labeled NB110-61640G
DyLight 680 Labeled NB110-61640FR
DyLight 755 Labeled NB110-61640IR
PE Labeled NB110-61640PE
PerCP Labeled NB110-61640PCP

Additional HSP90AB1 Antibody (H90-10) Products

HSP90AB1 NB110-61640

Related Products by Gene

Bioinformatics Tool for HSP90AB1 Antibody (NB110-61640)

Discover related pathways, diseases and genes to HSP90AB1 Antibody (NB110-61640). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HSP90AB1 Antibody (NB110-61640)

Discover more about diseases related to HSP90AB1 Antibody (NB110-61640).
 

Pathways for HSP90AB1 Antibody (NB110-61640)

View related products by pathway.

PTMs for HSP90AB1 Antibody (NB110-61640)

Learn more about PTMs related to HSP90AB1 Antibody (NB110-61640).

Blogs on HSP90AB1

There are no specific blogs for HSP90AB1, but you can read our latest blog posts.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol HSP90AB1

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB110-61640 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought