HSP90AB1 Antibody (H90-10)

Images

 
Western Blot: HSP90AB1 Antibody (H90-10) [NB110-61640] - HCC827 human non-small cell lung cancer cell line and A549 cells lysates. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: HSP90AB1 Antibody (H90-10) [NB110-61640] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal ...read more
Immunohistochemistry-Paraffin: HSP90AB1 Antibody (H90-10) [NB110-61640] - Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4C. ...read more
Flow Cytometry: HSP90AB1 Antibody (H90-10) [NB110-61640] - An intracellular stain was performed on Jurkat cells with antibody NB110-61640APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Western Blot: HSP90AB1 Antibody (H90-10) [NB110-61640] - Human cell lysates from various cell lines showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. 15 ug protein each lane. ...read more
Western Blot: HSP90AB1 Antibody (H90-10) [NB110-61640] - Human HeLa cell lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Primary Antibody: Mouse Anti-Hsp90 Monoclonal ...read more
Western Blot: HSP90AB1 Antibody (H90-10) [NB110-61640] - Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody ...read more
Immunocytochemistry/ Immunofluorescence: HSP90AB1 Antibody (H90-10) [NB110-61640] - Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 ...read more
Immunohistochemistry-Paraffin: HSP90AB1 Antibody (H90-10) [NB110-61640] - Cancerous human colon tissue.
Immunohistochemistry: HSP90AB1 Antibody (H90-10) [NB110-61640] - Mouse inflammed colon tissue. Fixation: Formalin. Primary antibody at 1:10000 for 12 hours at 4C. Secondary antibody: Alexa Fluor 555 Goat Anti-Mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
Clone
H90-10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

HSP90AB1 Antibody (H90-10) Summary

Immunogen
Recombinant human HSP90beta
Localization
Cytoplasm, Melanosome
Marker
ER Stress Marker
Specificity
Detects 90 kDa protein. Detects HSP90 beta in all reactive species except in Chicken, where it detects both alpha and beta isoforms.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HSP90AB1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
1 ug/ml of HSP90 antibody was sufficient for detection of HSP90beta in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Reviewed Applications
Read 1 Review rated 5
using
NB110-61640 in the following applications:

Reactivity Notes

Based on homology, it's predicted to detect Mouse, Rat, Rabbit, Chicken, Canine, Fish, White Sucker Fish (Catostomus commersonii) Shark Gummy Shark (Mustelus antarcticus) and School Shark (Galeorhinus galeus).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for HSP90AB1 Antibody (H90-10)

  • D6S182
  • Heat shock 84 kDa
  • heat shock 90kD protein 1, beta
  • heat shock 90kDa protein 1, beta
  • Heat Shock Protein 90 Alpha Family Class B Member 1
  • Heat Shock Protein 90 KDa
  • heat shock protein 90kDa alpha (cytosolic), class B member 1
  • Heat Shock Protein 90kDa Alpha Family Class B Member 1
  • heat shock protein HSP 90-beta
  • HSP 84
  • HSP 90
  • HSP84
  • HSP90B
  • HSP90-BETA
  • HSPC2
  • HSPCB

Background

HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms alpha and beta, which share 85% sequence amino acid homology. The two isoforms of Hsp90, are expressed in the cytosolic compartment (1). Despite the similarities, HSP90alpha exists predominantly as a homodimer while HSP90beta; exists mainly as a monomer.(2) From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex. (3-6) Furthermore, Hsp90 is highly conserved between species; having 60% and 78% amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively. Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite it's label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1'2% ofcytosolic protein). It carries out a number of housekeeping functions including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90- regulated proteins that have been discovered to date are involved in cell signaling. (7-8). The number of proteins now know to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5 When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function (9).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSP90AB1 Antibody (NB110-61640) (0)

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Review for HSP90AB1 Antibody (NB110-61640) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB110-61640:
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Images Ratings Applications Species Date Details
Western Blot HSP90AB1 NB110-61640
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reviewed by:
Anonymous
WB Human 10/17/2018
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Summary

ApplicationWestern Blot
Sample TestedHCC827 human non-small cell lung cancer cell line,A549 cells
SpeciesHuman
Lot0811

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSP90AB1 Antibody (NB110-61640) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-61640AF405
Alexa Fluor 488 Labeled NB110-61640AF488
Alexa Fluor 647 Labeled NB110-61640AF647
Alexa Fluor 700 Labeled NB110-61640AF700
Allophycocyanin Labeled NB110-61640APC
DyLight 350 Labeled NB110-61640UV
DyLight 405 Labeled NB110-61640V
DyLight 488 Labeled NB110-61640G
DyLight 680 Labeled NB110-61640FR
DyLight 755 Labeled NB110-61640IR
PE Labeled NB110-61640PE

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Recent Reviews

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2
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1
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Anonymous
10/17/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol HSP90AB1