Western blot analysis (1-2ug/ml), immunoprecipitation (4-8 ug/ml) and Immunohistochemistry (10-20 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes a 90 kDa protein, corresponding to the apparent molecular weight of Hsp90 on SDS-PAGE immunoblots. This antibody detects both Hsp90 alpha and beta.
90 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS, 1.0 mg/ml BSA and 50% Glycerol
Protein A or G purified
Alternate Names for HSP90 Antibody (S88)
Heat shock 86 kDa
heat shock 90kD protein 1, alpha
heat shock 90kD protein 1, alpha-like 4
heat shock 90kD protein, alpha-like 4
heat shock 90kDa protein 1, alpha
heat shock protein 90kDa alpha (cytosolic), class A member 1
heat shock protein HSP 90-alpha
Renal carcinoma antigen NY-REN-38
In mammalian cells there are at least two Hsp90 isoforms, Hsp90a and Hsp90b which are encoded by separate genes. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 is part of the cells powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by nonphysiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steriod receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90. It has been suggested that Hsp90 acts as a capacitor for morphological evolution by buffering widespread variation, which may affect morphogenic pathways.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for HSP90 Antibody (NB120-1429). (Showing 1 - 1 of 1 FAQs).
We were wondering how many western blots could be performed using your recommended dilutions and 0.05 mg of this antibody.
The number of Western Blots you will be able to perform with 0.05mg of antibody can vary greatly from product to product. We have a number of HSP90 antibodies to choose from, but one I see that is supplied as 0.05mg is NB120-1429. We recommend using 1-2ug per milliliter of diluent for this antibody. Unfortunately, I cannot give you an exact answer because it depends on the amount of diluent you will be using. Typically, our customer can get a good number of blots using our products.
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