HSP90 alpha Antibody (3B5)

Images

 
Western Blot: Hsp90A Antibody (3B5) [NBP2-02283] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Hsp90A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: Hsp90A Antibody (3B5) [NBP2-02283] Staining of paraffin-embedded prostate tissue using anti-Hsp90Amouse monoclonal antibody.
Immunohistochemistry-Paraffin: Hsp90A Antibody (3B5) [NBP2-02283] - Staining of paraffin-embedded liver tissue using anti-Hsp90Amouse monoclonal antibody.
Immunohistochemistry-Paraffin: Hsp90A Antibody (3B5) [NBP2-02283] - Staining of paraffin-embedded pancreas tissue using anti-Hsp90Amouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clone
3B5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HSP90 alpha Antibody (3B5) Summary

Immunogen
Full length human recombinant protein of human HSP90AA1 (NP_005339) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSP90AA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HSP90 alpha Antibody (3B5)

  • FLJ31884
  • Heat shock 86 kDa
  • heat shock 90kD protein 1, alpha
  • heat shock 90kD protein 1, alpha-like 4
  • heat shock 90kD protein, alpha-like 4
  • heat shock 90kDa protein 1, alpha
  • heat shock protein 90kDa alpha (cytosolic), class A member 1
  • heat shock protein HSP 90-alpha
  • HSP86
  • Hsp89
  • HSP89A
  • HSP90 alpha
  • Hsp90
  • HSP90A
  • HSP90AA1
  • HSP90AHSP86
  • HSP90N
  • HSPC1
  • HSPC1HSP 86
  • HSPCA
  • HSPCAHSP89A
  • HSPCAL1
  • HSPCAL4
  • HSPN
  • LAP2
  • Renal carcinoma antigen NY-REN-38

Background

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding,protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress.There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 (MIM 140572), a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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WB Video Protocol

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Secondary Antibodies

 

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Blogs on HSP90 alpha.

Hsp90A - helping to fold a wide variety of signal transduction proteins
Heat-shock protein 90 (Hsp90) is a conserved ATP-dependent chaperone with diverse cellular functions. Hsp90 is ubiquitously expressed but is further induced upon cellular stress such as heat-shock or nutrient deprivation. Hsp90 is essential for the...  Read full blog post.

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Bioinformatics

Gene Symbol HSP90AA1
Entrez