HSP70/HSPA1A Antibody

Images

 
Western Blot: HSP70/HSPA1A Antibody [NBP1-35969] - Analysis of 293T cell lysate.
Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody [NBP1-35969] - Human breast carcinoma

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price

HSP70/HSPA1A Antibody Summary

Immunogen
Synthetic peptide corresponding to aa 574-591 of human HSP70.
Specificity
This product is specific for Heat Shock Protein 70/hsp70
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPA1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.6) and 1.0% BSA
Preservative
0.1% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HSP70/HSPA1A Antibody

  • dnaK-type molecular chaperone HSP70-1
  • FLJ54303
  • FLJ54370
  • FLJ54392
  • FLJ54408
  • FLJ75127
  • Heat shock 70 kDa protein 1/2
  • heat shock 70 kDa protein 1A/1B
  • heat shock 70kD protein 1A
  • heat shock 70kDa protein 1A
  • heat shock-induced protein
  • HSP70
  • HSP70.1/HSP70.2
  • HSP70-1
  • HSP70-1/HSP70-2
  • HSP70-1A
  • HSP70I
  • HSP72
  • HSPA1
  • HSPA1A
  • HSPA1B

Background

Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the Cterminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HSPA1A
Entrez