HSP70/HSPA1A Antibody (5F3)

Images

 
Western Blot: Hsp70 Antibody (5F3) [NBP2-02189] Analysis of extracts (35ug) from 9 different cell lines by using anti-Hsp70 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Hsp70 Antibody (5F3) [NBP2-02189] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hsp70.
Flow Cytometry: Hsp70 Antibody (5F3) [NBP2-02189] - Analysis of Jurkat cells, using anti-Hsp70 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Western Blot: Hsp70 Antibody (5F3) [NBP2-02189] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Hsp70 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: Hsp70 Antibody (5F3) [NBP2-02189] - Immunofluorescent staining of HepG2 cells using anti-Hsp70 mouse monoclonal antibody.
Flow Cytometry: Hsp70 Antibody (5F3) [NBP2-02189] - Analysis of Hela cells, using anti-Hsp70 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-02189
    • Availability
      Product Discontinued

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HSP70/HSPA1A Antibody (5F3) Summary

Immunogen
Full length human recombinant protein of human HSPA1A (NP_005336) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSPA1A
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:4000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100

Reactivity Notes

Human, Dog, Monkey

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HSP70/HSPA1A Antibody (5F3)

  • dnaK-type molecular chaperone HSP70-1
  • FLJ54303
  • FLJ54370
  • FLJ54392
  • FLJ54408
  • FLJ75127
  • Heat shock 70 kDa protein 1/2
  • heat shock 70 kDa protein 1A/1B
  • heat shock 70kD protein 1A
  • heat shock 70kDa protein 1A
  • heat shock-induced protein
  • HSP70
  • HSP70.1/HSP70.2
  • HSP70-1
  • HSP70-1/HSP70-2
  • HSP70-1A
  • HSP70I
  • HSP72
  • HSPA1
  • HSPA1A
  • HSPA1B

Background

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HSPA1A
Entrez