HSP70/HSPA1A Antibody (13D3)

Images

 
Western Blot: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP70 monoclonal antibody at a ...read more
Immunocytochemistry/ Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Analysis of HSC70 using Monoclonal antibody (13D3) shows staining in NIH-3T3 cells. HSC70 staining (green), F-Actin staining with ...read more
Immunohistochemistry-Paraffin: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Both normal and cancer biopsies of deparaffinized human Prostate carcinoma tissue.
Immunocytochemistry/ Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker ...read more
Immunocytochemistry/ Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Analysis of HSC70 using HSC70 Monoclonal antibody (13D3) shows staining in HeLa cells. HSC70 staining (green), F-Actin staining with ...read more
Immunocytochemistry/ Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Analysis of HSC70 using HSC70 Monoclonal antibody (13D3) shows staining in MCF-7 cells. HSC70 staining (green), F-Actin staining with ...read more
Immunohistochemistry-Paraffin: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Both normal and cancer biopsies of deparaffinized human Tonsil tissue.
Immunohistochemistry-Paraffin: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Both normal and cancer biopsies of deparaffinized human Testis tissue.
Immunoprecipitation: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Analysis of HSC70 was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 500ug whole cell lysate with 2ul of HSC70 monoclonal ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Fe, Fi, Ha, PmSpecies Glossary
Applications WB, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N
Clone
13D3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HSP70/HSPA1A Antibody (13D3) Summary

Immunogen
Mouse spermatogenic cell protein.
Specificity
This displays slight cross-reactivity to HSP 70.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
HSPA1A
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:5000
  • Flow Cytometry 1:10 - 1:1000
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:20 - 1:200
  • Immunohistochemistry 1:20 - 1:200
  • Immunohistochemistry-Frozen 1:20 - 1:200
  • Immunohistochemistry-Paraffin 1:20 - 1:200
  • Immunoprecipitation 2 ul
  • Block/Neutralize
Application Notes
WB: Detects an approx. 70 kDa protein representing Hsc 70. By 2D gel electrophoresis, this antibody binds strongly to Hsc 70 and faintly to HSP 70 both before and after heat shock. IHC: Staining of Hsc 70 in mouse spermatids/spermatozoa results in staining restricted to the post acrosomal region in condensing spermatids and to the midpiece in spermatozoa. May be useful in IHC-Fr. Use in Immunoblotting reported in scientific literature (PMID 28095639).
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 3.5
using
NB120-2788 in the following applications:

Publications
Read Publications using
NB120-2788 in the following applications:

  • 1 publication
  • IB
    1 publication
  • 2 publications
  • IP
    2 publications
  • WB
    5 publications

Reactivity Notes

Reported cross-reactivity with Bovine in literature (Terlecky et al.). Hamster reactivity reported in scientific literature (PMID: 16737757). Fish reactivity reported in scientific literature (PMID: 9006893). Rat reactivity reported by customer review. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites diluted with PBS
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for HSP70/HSPA1A Antibody (13D3)

  • dnaK-type molecular chaperone HSP70-1
  • FLJ54303
  • FLJ54370
  • FLJ54392
  • FLJ54408
  • FLJ75127
  • Heat shock 70 kDa protein 1/2
  • heat shock 70 kDa protein 1A/1B
  • heat shock 70kD protein 1A
  • heat shock 70kDa protein 1A
  • heat shock-induced protein
  • HSP70
  • HSP70.1/HSP70.2
  • HSP70-1
  • HSP70-1/HSP70-2
  • HSP70-1A
  • HSP70I
  • HSP72
  • HSPA1
  • HSPA1A
  • HSPA1B

Background

Heat shock proteins (HSP's) or stress response proteins (SRP's) are synthesized in variety of environmental and pathophysiological stressful conditions. Many HSP's are involved in processes such as protein denaturation-renaturation, folding-unfolding, transport-translocation, activation-inactivation, and secretion. HSP70 is found to be associated with steroid receptors,actin, p53, polyoma T antigen, nucleotides, and other unknown proteins. Also, HSP70 has been shown to be involved in protective roles against thermal stress, cytotoxic drugs, and other damaging conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSP70/HSPA1A Antibody (NB120-2788)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: FLOW, IB, ICC/IF, IP, WB.


Filter By Application
FLOW
(1)
IB
(1)
ICC/IF
(2)
IP
(2)
WB
(5)
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Filter By Species
Human
(2)
Mouse
(1)
Rat
(2)
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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB120-2788 Applications Species
Pajares M, Rojo AI, Arias E et al. Transcription factor NFE2L2/NRF2 modulates chaperone-mediated autophagy through the regulation of LAMP2A Autophagy Jul 26 2018 [PMID: 29950142] (WB, Human) WB Human
Gong Z, Tasset I, Diaz A et al. Humanin is an endogenous activator of chaperone-mediated autophagy J. Cell Biol. Feb 5 2018 [PMID: 29187525] (WB, Rat) WB Rat
Zhang S, Eitan E, Mattson MP. Early Involvement of Lysosome Dysfunction in the Degeneration of Cerebral Cortical Neurons Caused by the Lipid Peroxidation Product 4-Hydroxynonenal J. Neurochem Jan 17 2017 [PMID: 28095639] (IB, Rat) IB Rat
Ganesan S, Reynolds C, Hollinger K et al. Twelve hours of heat stress induces inflammatory signaling in porcine skeletal muscle. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2016 Mar 23 [PMID: 27009052]
Park C, Suh Y, Cuervo AM. Regulated degradation of Chk1 by chaperone-mediated autophagy in response to DNA damage Nat Commun 2015 Apr 17 [PMID: 25880015] (WB, ICC/IF, IP, Mouse) WB, ICC/IF, IP Mouse
Kett LR, Stiller B, Bernath MM et al. alpha-Synuclein-Independent Histopathological and Motor Deficits in Mice Lacking the Endolysosomal Parkinsonism Protein Atp13a2 J. Neurosci. 2015 Apr 08 [PMID: 25855184]
Ferreira JV, Fofo H, Bejarano E et al. STUB1/CHIP is required for HIF1A degradation by chaperone-mediated autophagy. Autophagy 2013 Jun 7 [PMID: 23880665]
Hubbi ME, Hu H, Kshitiz F et al. Chaperone-mediated autophagy targets HIF-1alpha for lysosomal degradation. J Biol Chem 2013 Mar 1 [PMID: 23457305]
Orenstein SJ, Kuo SH, Tasset I et al. Interplay of LRRK2 with chaperone-mediated autophagy. Nat Neurosci 2013 Apr [PMID: 23455607] (WB, Human) WB Human
H Koga, S KaushikA M Cuervo. Altered lipid content inhibits autophagic vesicular fusion. FASEB J; fj09-144519. 2010 [PMID: 20375270]
Show All 14 Publications.

Reviews for HSP70/HSPA1A Antibody (NB120-2788) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB120-2788:
Filter by Applications
Simple Western
(1)
WB
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western HSP70/HSPA1A NB120-2788
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reviewed by:
Anonymous
Simple Western Mouse 01/21/2016
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Summary

ApplicationSimple Western
Sample Testedwhole brain homogenates from mice
SpeciesMouse

Blocking

Blocking Detailsn/a

Primary Anitbody

Dilution Ratio1:2000, 1:500, 1:100

Details

Detection NotesWES Simple Western platform

Comments

CommentsIt works better at low concentrations.
 
reviewed by:
Anonymous
WB Human 02/04/2009
View

Summary

ApplicationWestern Blot
Sample TestedHEK293 cell lysate, Sample Amount: 20 ug
SpeciesHuman
Lot056-114

Blocking

Blocking DetailsBlocking Buffer: Li-Cor blocking buffer, Blocking Time: 30 minutes, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1/10000, Incubation Dilution Buffer: Li-Cor blocking buffer, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: Alexa 680 goat anti-mouse pAb, Secondary Ab Dilution Ratio: 1/25000

Details

Detection NotesDetection Method: Infra-red imaging, Specific Bands: 70 kDa, Non-Specific Bands: 40 kDa

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSP70/HSPA1A Antibody (NB120-2788). (Showing 1 - 2 of 2 FAQs).

  1. What is the concentration of this product?
    • As this product is sold as unpurified ascites, the concentration of the antibody has not been determined.
  2. Would any endotoxin and azide exist in buffer?
    • The antibody solution contains 0.05% sodium azide. We have not tested this product for endotoxin levels.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

3.5
5
0
4
1
3
1
2
0
1
0

Anonymous
01/21/2016
Application: Simple Western
Species: Mouse

 
Anonymous
02/04/2009
Application: WB
Species: Human

Bioinformatics

Gene Symbol HSPA1A
Entrez
Uniprot