Hsp70 / Hsc70 Antibody (N27F3-4) [Alkaline Phosphatase]

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Western Blot: Hsp70 / Hsc70 Antibody (N27F3-4) [Alkaline Phosphatase] [NBP1-97879] - Analysis of monkey Vero cell lysate

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-97879
    • Availability
      Product Discontinued

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Hsp70 / Hsc70 Antibody (N27F3-4) [Alkaline Phosphatase] Summary

Immunogen
Purified Hsc70 and Hsp70 isolated from human HeLa cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSPA1A
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Application Notes
This antibody is useful in Flow Cytometry, Immunohistochemistry (paraffin sections), Immunoprecipitation and detects a band of ~72-73kDa by Western blot.
Theoretical MW
72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NBP1-97879.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
100 mM Tris (pH 7.6), 150 mM NaCl, 1 mM Magnesium Chloride, and 0.1 mM Zinc
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for Hsp70 / Hsc70 Antibody (N27F3-4) [Alkaline Phosphatase]

  • heat shock 70kDa protein 1A
  • HSP70-1
  • HSP70-1A
  • HSP70I
  • HSP72
  • HSPA1
  • HSPA1A

Background

The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Hsp70 / Hsc70 Antibody (NBP1-97879)(2)

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Product General Protocols

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Video Protocols

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FAQs for Hsp70 / Hsc70 Antibody (NBP1-97879) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for Hsp70 / Hsc70 Antibody (NBP1-97879)

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Blogs on Hsp70 / Hsc70.

Hsp70/Hsc70 - A chaperone for protein folding and autophagy
The heat shock protein 70 (Hsp70) family are a group of chaperones involved in various cellular processes including protein folding and protein degradation. In addition to maintaining normal cellular homeostasis by processing newly synthesized pro...  Read full blog post.

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Bioinformatics

Gene Symbol HSPA1A