Hsp70 / Hsc70 Antibody (N27F3-4)

Images

 
Western Blot: Hsp70 / Hsc70 Antibody (N27F3-4) [NB110-96425] - analysis of Human Cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27. Load: 15 ug protein. Block: 1.5% ...read more
Immunohistochemistry-Paraffin: Hsp70 / Hsc70 Antibody (N27F3-4) [NB110-96425] - analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, EM, Flow, ICC/IF, IHC, IHC-P, IM, IP
Clone
N27F3-4
Clonality
Monoclonal
Host
Mouse
Concentration
1 mg/ml

Hsp70 / Hsc70 Antibody (N27F3-4) Summary

Immunogen
Detects 72 and 73kDa proteins corresponding to the molecular mass of inducible Hsp and Hsc on SDS PAGE immunoblots.
Localization
Cytoplasm
Specificity
Detects approx 72 (HSP) and approx 73kDa (HSC).
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
HSPA1A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Electron Microscopy 1:10-1:500
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunomicroscopy
  • Immunoprecipitation 1:10-1:500
Application Notes
1 ug/ml of HSP70/HSC70 Antibody was sufficient for detection of HSP70/HSC70 in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Reactivity Notes

Based on homology, It is predicted to detect Mouse, Rat, Bovine, Nematode (Caenorhabditis elegans), Beluga, Canine, Chicken, Drosophila, Fish, Guinea Pig, Hamster, Monkey, Porcine, Plant, Cucumber, Pea (Pisum sativum), Rabbit, Sheep and Xenopus

Alternate Names for Hsp70 / Hsc70 Antibody (N27F3-4)

  • heat shock 70kDa protein 1A
  • HSP70-1
  • HSP70-1A
  • HSP70I
  • HSP72
  • HSPA1
  • HSPA1A

Background

Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eucaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eucaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity. The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins. All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein. The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Hsp70 / Hsc70 Antibody (NB110-96425) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-96425AF405
Alexa Fluor 488 Labeled NB110-96425AF488
Alexa Fluor 647 Labeled NB110-96425AF647
Alexa Fluor 700 Labeled NB110-96425AF700
Allophycocyanin Labeled NB110-96425APC
DyLight 350 Labeled NB110-96425UV
DyLight 405 Labeled NB110-96425V
DyLight 405LS Labeled NB110-96425V3
DyLight 488 Labeled NB110-96425G
DyLight 680 Labeled NB110-96425FR
DyLight 755 Labeled NB110-96425IR
PE Labeled NB110-96425PE
PerCP Labeled NB110-96425PCP

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Blogs on Hsp70 / Hsc70.

Hsp70/Hsc70 - A chaperone for protein folding and autophagy
The heat shock protein 70 (Hsp70) family are a group of chaperones involved in various cellular processes including protein folding and protein degradation. In addition to maintaining normal cellular homeostasis by processing newly synthesized pro...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol HSPA1A
Entrez
Uniprot

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