HSP60 Antibody (A57-E4)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB120-5486
    • Availability
      Product Discontinued

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HSP60 Antibody (A57-E4) Summary

Immunogen
Recombinant Chlamydia trachomatis HSP60.
Epitope
Epitope mapping studies suggest that this antibody binds to a linear sequence of 6 amino acids of Chlamydia Hsp 60 amino acids 117-122.
Localization
Mitochondrial/chloroplast
Marker
Mitochondria Marker
Specificity
Detects heat shock protein 60 kDa (Hsp 60) from bacteria. This detects Hsp 60 from all three species of Chlamydia, Escherichia coli (GroEL), Salmonella typhimurium, Neisseria gonorrhoeae and Coxiella burnetti. ab5486 does not cross react with HeLa cell HSP60.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSPD1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
Application Notes
WB: Use at a dilution of 1/5000. Detects an approx. 60 kDa representing Hsp 60 from C. trachomatis lysate

Reactivity Notes

E. coli and Salmonella typhi.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites with PBS
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for HSP60 Antibody (A57-E4)

  • 60 kDa chaperonin
  • Chaperonin 60
  • cpn60
  • GROEL
  • heat shock 60kD protein 1 (chaperonin)
  • heat shock 60kDa protein 1 (chaperonin)
  • Heat shock protein 60
  • heat shock protein 65
  • HLD4
  • HSP60
  • HSP-60
  • HSP60SPG13
  • HSP65
  • HSPD1
  • HuCHA60
  • Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial
  • P60 lymphocyte protein
  • short heat shock protein 60 Hsp60s1
  • spastic paraplegia 13 (autosomal dominant)
  • SPG13

Background

Heat shock proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP70, HSP90 and HSP100. HSP60, an ~60 kDa protein, is the human homologue of HSP65 (a major antigenic mycobacterial protein) and E. coli GroEL. HSP60, also referred to as chaperonin-60, P1 & mitonin, has been classified as a member of a family of proteins termed chaperonins which act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly. HSP60 is a constitutively expressed mitochondrial protein that shares many characteristics with HSP70 including high abundance, induction by environmental stress, ATPase activity and a critical role in protein assembly and folding. However, in contrast with HSP70, HSP60 functions as a 14 subunit oligomer which is thought to interact with only 1 or 2 target polypeptides at one time.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Research Areas for HSP60 Antibody (NB120-5486)

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Blogs on HSP60.

TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

Customer Experience using HSP60 Antibody
I began using the HSP60 antibody (NB110-57063) in June of 2010 and it worked well. I do not like to buy antibodies that have not been tested in the species for which I will use them, so I picked this antibody because it had already been tested in rat...  Read full blog post.

Heat Shock Proteins: An Overview
Heat Shock Proteins (HSPs) are a ubiquitous group of molecular chaperone proteins that have evolved unique mechanisms, within their host cells, to facilitate survival in hostile environments such as heat, oxidative (hypoxia), pH and cold. Under permis...  Read full blog post.

The Heat is On: Heat Shock Proteins and the Link to Cancer
Novus Biologicals offers an extensive antibody catalog targeting heat shock proteins (HSPs). A large protein group covering a number of families, the HSPs are functionally related by their dramatic upregulation in response to stress. Stress triggers m...  Read full blog post.

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Bioinformatics

Gene Symbol HSPD1
Entrez