HSP60 Antibody (A57-E4)



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HSP60 Antibody (A57-E4) Summary

Recombinant Chlamydia trachomatis Hsp 60.
Epitope mapping studies suggest that this antibody binds to a linear sequence of 6 amino acids of Chlamydia Hsp 60 amino acids 117-122.
Mitochondria Marker
Detects heat shock protein 60 kDa (Hsp 60) from bacteria. This detects Hsp 60 from all three species of Chlamydia, Escherichia coli (GroEL), Salmonella typhimurium, Neisseria gonorrhoeae and Coxiella burnetti. ab5486 does not cross react with HeLa cell HSP60.
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Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Ascites with PBS
0.05% Sodium Azide


Application Notes
WB: Use at a dilution of 1/5000. Detects a band of approximately 60 kDa representing Hsp 60 from C. trachomatis lysate.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with E. coli and Salmonella typhi.Not yet tested in other species.


Heat shock proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP70, HSP90 and HSP100. HSP60, an ~60 kDa protein, is the human homologue of HSP65 (a major antigenic mycobacterial protein) and E. coli GroEL. HSP60, also referred to as chaperonin-60, P1 & mitonin, has been classified as a member of a family of proteins termed chaperonins which act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly. HSP60 is a constitutively expressed mitochondrial protein that shares many characteristics with HSP70 including high abundance, induction by environmental stress, ATPase activity and a critical role in protein assembly and folding. However, in contrast with HSP70, HSP60 functions as a 14 subunit oligomer which is thought to interact with only 1 or 2 target polypeptides at one time.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product PDFs


Gene Symbol HSPD1

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