HSP60 Antibody (2E4)

Images

 
Western Blot: Hsp60 Antibody (2E4) [NBP1-04303] - Cell lysates of Hela (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human HSP60 (1:1000). Proteins were visualized using a goat ...read more
Immunocytochemistry/ Immunofluorescence: HSP60 Antibody (2E4) [NBP1-04303] - Analysis in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HSP60 antibody (1:100) with goat ...read more
Immunohistochemistry-Paraffin: Hsp60 Antibody (2E4) [NBP1-04303] - Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp60 (1:50) for 2 hours at room temperature. Antigen retrieval ...read more
Flow Cytometry: HSP60 Antibody (2E4) [NBP1-04303] - Flow cytometry analysis of HSP60 in A549 cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat antimouse IgG Alexa fluor 488 conjugate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-P
Clone
2E4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

HSP60 Antibody (2E4) Summary

Immunogen
Recombinant human HSP60 (1-573aa) purified from E. coli
Marker
Mitochondria Marker
Specificity
Anti-human HSP60 mAb, clone 2E4, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HSP60 protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HSPD1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 2-5ug - 1-10-6-cells
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry-Paraffin 1:50-100

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for HSP60 Antibody (2E4)

  • 60 kDa chaperonin
  • Chaperonin 60
  • cpn60
  • GROEL
  • heat shock 60kD protein 1 (chaperonin)
  • heat shock 60kDa protein 1 (chaperonin)
  • Heat shock protein 60
  • heat shock protein 65
  • HLD4
  • HSP60
  • HSP-60
  • HSP60SPG13
  • HSP65
  • HSPD1
  • HuCHA60
  • Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial
  • P60 lymphocyte protein
  • short heat shock protein 60 Hsp60s1
  • spastic paraplegia 13 (autosomal dominant)
  • SPG13

Background

Heat shock protein 60 (HSP60) is a mitochondrial chaperonin that is typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. HSP60 is the approximate 60kDa mammalian equivalent to GroEL of E.coli. Process of HSP60 is regulated by the cochaperonin HSP10, a single heptameric ring of approximately 10kD subunits that forms a complex with HSP60. HSP10 coordinates the ATPase activity of the HSP60 subunits to allow the release of bound polypeptide in a manner productive for folding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

Customer Experience using HSP60 Antibody
I began using the HSP60 antibody (NB110-57063) in June of 2010 and it worked well. I do not like to buy antibodies that have not been tested in the species for which I will use them, so I picked this antibody because it had already been tested in rat...  Read full blog post.

Heat Shock Proteins: An Overview
Heat Shock Proteins (HSPs) are a ubiquitous group of molecular chaperone proteins that have evolved unique mechanisms, within their host cells, to facilitate survival in hostile environments such as heat, oxidative (hypoxia), pH and cold. Under permis...  Read full blog post.

The Heat is On: Heat Shock Proteins and the Link to Cancer
Novus Biologicals offers an extensive antibody catalog targeting heat shock proteins (HSPs). A large protein group covering a number of families, the HSPs are functionally related by their dramatic upregulation in response to stress. Stress triggers m...  Read full blog post.

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Bioinformatics

Gene Symbol HSPD1
Entrez
Uniprot