Hsp47 Antibody

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Western Blot: Hsp47 Antibody [NBP1-45627] - Analysis of Hsp47 expression in mouse small intestine tissue lysate.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-45627
    • Availability
      Product Discontinued

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Hsp47 Antibody Summary

Immunogen
Synthetic peptide surrounding amino acid 407 of human HSP47.
Clonality
Polyclonal
Host
Rabbit
Gene
SERPINH1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
Application Notes
Western blotting (0.5-4 ug/ml), immunoprecipitation (10-20 ug/ml) and Immunohistochemistry (10-20 ug/ml) in frozen or paraffin embedded tissues. However, the optimal concentrations should be determined individually. The antibody recognizes 47 kDa Hsp47 of human, mouse, rat, and chick origins. Mouse small intestine tissue lysate can be used as a positive control.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.5% BSA and 30% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.5 mg/ml
Purity
Protein A purified

Alternate Names for Hsp47 Antibody

  • (collagen binding protein 1)
  • Arsenic-transactivated protein 3
  • AsTP3
  • BERF-1
  • CBP1
  • CBP2
  • CBP2RA-A47
  • Cell proliferation-inducing gene 14 protein
  • Collagen-binding protein
  • colligen
  • Colligin
  • colligin-1
  • colligin-2
  • gp46
  • HSP47
  • HSP47serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47)
  • member 1, (collagen binding protein 1)
  • member 2
  • member 2, (collagen-binding protein 2)
  • OI10
  • PPROM
  • proliferation-inducing gene 14
  • RA-A47
  • rheumatoid arthritis antigen A-47,47 kDa heat shock protein
  • Rheumatoid arthritis-related antigen RA-A47
  • serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47)
  • Serpin H1
  • serpin peptidase inhibitor, clade H (heat shock protein 47), member 1
  • SERPINH1
  • SERPINH2colligin

Background

This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SERPINH1
Entrez
Uniprot