HSP40/DNAJB1 Antibody

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Western Blot: HSP40/DNAJB1 Antibody [NB100-91818] - Analysis of HSP40 (P303) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate(20ug)Lane2:RAW264.7 whole cell lysate(20ug)Lane3:H9C2 whole cell lysate(20ug)
Immunohistochemistry-Paraffin: HSP40/DNAJB1 Antibody [NB100-91818] - Paraffin-embedded human breast carcinoma tissue at 1:100
Western Blot: Hsp40/DNAJB1 Antibody [NB100-91818] - - Western blot (WB) analysis of HSP40/HDJ-1 (P303) antibody in extracts from COLO205 cells.
Western Blot: Hsp40/DNAJB1 Antibody [NB100-91818] - Lane1:Hela cell lysate treated with UV. Lane2:HEK293T cell lysate treated with UV. Lane3:NIH-3T3 cell lysate treated with UV. Lane4:PC12 cell lysate treated with UV.
Immunohistochemistry: Hsp40/DNAJB1 Antibody [NB100-91818] - - Immunohistochemistry (IHC) analyzes of HSP40/HDJ-1 (P303) antibody in paraffin-embedded lung carcinoma tissue

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-91818
    • Availability
      Product Discontinued

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HSP40/DNAJB1 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 270-320 of Human HSP40.
Specificity
HSP40/HDJ-1 (P303) detects endogenous levels of HSP40/HDJ-1 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
DNAJB1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of HSP40/HDJ-1 (P303) pAb in extracts from COLO205 cells.
Immunohistochemistry (IHC) analyzes of HSP40/HDJ-1 (P303) pAb in paraffin-embedded lung carcinoma tissue.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HSP40/DNAJB1 Antibody

  • DnaJ (Hsp40) homolog, subfamily B, member 1
  • DnaJ (Hsp40) homolog, subfmaily B, member 1
  • dnaJ homolog subfamily B member 1
  • DnaJ protein homolog 1
  • DNAJ1
  • DNAJB1
  • Hdj1
  • hDj-1
  • Heat shock 40 kDa protein 1
  • heat shock 40kD protein 1
  • Heat shock protein 40
  • HSP40
  • HSPF1
  • HSPF1Hdj1
  • Human DnaJ protein 1
  • radial spoke 16 homolog B
  • RSPH16B
  • Sis1

Background

Heat shock protein 40 (HSP 40) family proteins bind to HSP 70 through their J-domain and regulate the function of HSP 70 by stimulating HSP 70 ATPase activity. HSP 40, also known as DnaJ, functions together with DnaK (HSP 70) and GrpE as a molecular chaperone, involving them in assembly and disassembly of protein complexes, protein folding, renaturation of denatured proteins, prevention of protein aggregation and protein export. HSP 40 stimulates the association between HSC 70 and HIP and translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. There are five known HSP 40 family proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol DNAJB1
Entrez