HSP27 [p Ser78] Antibody

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Western Blot: Hsp27 Antibody [NBP1-51455] - Extracts from HeLa Ca2+ cells.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-51455
    • Availability
      Product Discontinued

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HSP27 [p Ser78] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human HSP27 around the phosphorylation site of Serine 78.
Modification
p Ser78
Specificity
p-HSP27 (S78) pAb detects endogenous levels of HSP27 protein only when phosphorylated at S78.
Clonality
Polyclonal
Host
Rabbit
Gene
HSPB1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
Application Notes
Western blot (WB) analyzes of p-HSP27 (S78) pAb in extracts from HeLa Ca2+ cells.
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HSP27 [p Ser78] Antibody

  • 28 kDa heat shock protein
  • DKFZp586P1322
  • Estrogen-regulated 24 kDa protein
  • Heat shock 27 kDa protein
  • heat shock 27kD protein 1
  • heat shock 27kDa protein 1
  • heat shock protein beta-1
  • HMN2B
  • HS.76067
  • HSP25
  • HSP27
  • HSP27HSP 27
  • HSP28CMT2F
  • HSPB1
  • SRP27
  • Stress-responsive protein 27

Background

HSP 27 is a constitutively expressed cytoplasmic protein that co-localizes to the nucleus upon stress-induced insult. The intracellular concentration of HSP 27 increases several-fold after heat shock and other metabolic stresses, and is closely associated with the acquisition of thermotolerance. In addition to heat shock, cytokines and hormones are among the factors that stimulate the synthesis of HSP 27. MAP kinase-activated protein kinase-2 phosphorylates HSP 27 on Serine residues Ser 15, Ser 78 and Ser 82, which are phosphorylated in vivo in response to growth factors and heat shock. Ser 15, Ser 78 and Ser 82 occur in the sequence motif RXXS, which is recognized by ribosomal protein S6 kinase II.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HSPB1
Entrez