Hsp104 Antibody



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Hsp104 Antibody Summary

Synthetic peptide: DDDNEDSMEIDDDLD, corresponding to amino acids 894-908 of S. cerevisiae Hsp104.
Cytoplasmic and Nuclear
Hsp 104
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Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Whole antisera with PBS
0.05% Sodium Azide


Application Notes
Immunocytochemistry: use at a dilution of 1/500 (Yeast). Western Blot: use at a dilution of 1/5000 (Yeast). Western Blot: use at a dilution of 1/100 (Mammalian). Immunoprecipitation: assay dependent. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a single ~104 kDa protein from yeast and 100 - 105 kDa proteins from mammalian sources. Immunofluorescence staining of HSP 104 in yeast results in punctate nuclear and cytoplasmic staining.

Reactivity Notes

Cross-reacts with Human, Mouse and S. cerevisiae. Does not cross-react with Drosophilia melanogaster, E. coli or Plants. Not yet tested in other species.


Heat Shock Proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP 70, HSP 90 and HSP 100. The HSP 100 proteins generally have amino acid sequences of about 900 residues and contain two nucleotide-binding sites. Within the HSP 100 family of proteins, yeast express an ~104 kDa form which is necessary to protect cells from various stress conditions such as heat, heavy metals and ethanol, though mutation studies have shown that the protein is not required for normal growth. Yeast HSP 104 has been shown to be a ClpB protein with significant sequence homology to E. coli ClpB, particularly in the two nucleotide-binding sites.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Additional Hsp104 Antibody Products

Hsp104 NB120-2924

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