Hsp104 Antibody

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB120-2924
    • Availability
      Product Discontinued

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Hsp104 Antibody Summary

Immunogen
Synthetic peptide: DDDNEDSMEIDDDLD, corresponding to amino acids 894-908 of S. cerevisiae Hsp104.
Localization
Cytoplasmic and Nuclear
Specificity
Hsp 104
Clonality
Polyclonal
Host
Rabbit
Purity
Unpurified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
Application Notes
Immunocytochemistry: use at a dilution of 1/500 (Yeast). Western Blot: use at a dilution of 1/5000 (Yeast). Western Blot: use at a dilution of 1/100 (Mammalian). Immunoprecipitation: assay dependent. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a single ~104 kDa protein from yeast and 100 - 105 kDa proteins from mammalian sources. Immunofluorescence staining of HSP 104 in yeast results in punctate nuclear and cytoplasmic staining.

Reactivity Notes

Cross-reacts with Human, Mouse and S. cerevisiae. Does not cross-react with Drosophilia melanogaster, E. coli or Plants. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera with PBS
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Hsp104 Antibody

  • Heat shock protein 104
  • Hsp 104
  • L0948

Background

Heat Shock Proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP 70, HSP 90 and HSP 100. The HSP 100 proteins generally have amino acid sequences of about 900 residues and contain two nucleotide-binding sites. Within the HSP 100 family of proteins, yeast express an ~104 kDa form which is necessary to protect cells from various stress conditions such as heat, heavy metals and ethanol, though mutation studies have shown that the protein is not required for normal growth. Yeast HSP 104 has been shown to be a ClpB protein with significant sequence homology to E. coli ClpB, particularly in the two nucleotide-binding sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Hsp104 Products

Hsp104 NB120-2924

Research Areas for Hsp104 Antibody (NB120-2924)

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