HSP10/EPF Antibody [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, A431 human epithelial carcinoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL Human/Mouse HSP10/EPF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3298) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for HSP10/EPF was detected at approximately 8-9 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 4.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

HSP10/EPF Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human HSP10/EPF
  Ala2-Asp102
  Accession # P61604
• Specificity: Detects endogenous human and mouse HSP10/EPF in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HSPE1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HSP10/EPF Antibody [Unconjugated]

• 10 kDa chaperonin
• 10 kDa heat shock protein, mitochondrial
• Chaperonin 10 Homolog
• Chaperonin 10
• CPN10HSP10
• Early-pregnancy factor
• EPF
• GroES
• heat shock 10kD protein 1 (chaperonin 10)
• heat shock 10kDa protein 1 (chaperonin 10)
• HSP10
• HSPE1

Background

The heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. HSP10, also known as HSPE1, chaperonin 10 and Early Pregnancy Factor (EPF), is a 10.8 kDa protein with an apparent molecular weight of 8‑9 kDa that exists intracellularly as a 7 subunit multimer and assists HSP60 in folding non-native proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSP10/EPF Antibody (AF3298)(1)

Publication using AF3298

• Anraku , Itaru, Rajasuriar , Reena, Dobbin , Caroline, Brown , Richard, Lewin , Sharon R, Suhrbier , Andreas Circulating heat shock protein 60 levels are elevated in HIV patients and are reduced by anti-retroviral therapy. PLoS ONE, 2012-09-28;7(9):e45291. 2012-09-28 [PMID: 23028910] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: HSPE1
• Uniprot:
  • P61604()