HSF2 Antibody (JE48-40)

Images

 
Western Blot: HSF2 Antibody (JE48-40) [NBP2-80457] - Analysis of CRIPTO on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary ...read more
Immunocytochemistry/ Immunofluorescence: HSF2 Antibody (JE48-40) [NBP2-80457] - Staining of HSF2 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room ...read more
Immunohistochemistry-Paraffin: HSF2 Antibody (JE48-40) [NBP2-80457] - Analysis in human gastric cancer tissue using CRIPTO antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate ...read more
Flow Cytometry: HSF2 Antibody (JE48-40) [NBP2-80457] - Analysis of HSF2 was done on 293 cells. The cells were fixed, permeabilized and stained with Carcino Embryonic Antigen CEA antibody at 1/100 dilution (red) compared ...read more
Immunohistochemistry-Paraffin: HSF2 Antibody (JE48-40) [NBP2-80457] - Analysis in human breast cancer tissue using CRIPTO antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate ...read more
Immunohistochemistry-Paraffin: HSF2 Antibody (JE48-40) [NBP2-80457] - Analysis in human prostate cancer tissue using CRIPTO antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate ...read more
Immunohistochemistry-Paraffin: HSF2 Antibody (JE48-40) [NBP2-80457] - Analysis in human skin tissue using CRIPTO antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH ...read more
Immunohistochemistry-Paraffin: HSF2 Antibody (JE48-40) [NBP2-80457] - Analysis in human colon cancer tissue using CRIPTO antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate ...read more

Product Details

Summary
Product Discontinued
View other related HSF2 Primary Antibodies

Order Details


    • Catalog Number
      NBP2-80457
    • Availability
      Product Discontinued

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HSF2 Antibody (JE48-40) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant protein within Human HSF2 aa 426-536 / 536. (SwissProt: Q03933 Human)
Localization
Nucleus, Cytoplasm.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
HSF2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for HSF2 Antibody (JE48-40)

  • heat shock factor 2
  • heat shock factor protein 2
  • heat shock transcription factor 2MGC75048
  • HSF 2
  • HSF2
  • HSTF 2
  • HSTF2
  • MGC117376
  • MGC156196

Background

Heat shock factor 1 (HSF1) is a heat shock transcription factor that activates the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function. Up-regulation of expression of heat shock proteins in response to stress occurs at the level of transcription through a heat shock element and a HSF transcription factor. Amino acid sequences for most HSFs are highly conserved. A DNA binding domain is at the N-terminus, hydrophobic repeats (essential to the formation of active trimers) are adjacent to this binding domain, and another short hydrophobic repeat (necessary for suppression of trimerization) occurs toward the C-terminus. HSF2 exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form. Various experiments have suggested that HSF2 may play roles in differentiation and development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HSF2