We first test this antibody in HEK293T (Human Embryonic Kidney Cells) and N2a (mouse neuroblastoma cell line) cell lysis. Both of them show the positive bands when NBP2-46005 is used. Then we do the IP experiments, the bait is the interacting protein of HSD17B4 coated beads which are incubated with N2a cell lysates, we also set the control which is free of any interacting protein of HSD17B4. It can be detected in the blot that input and bait both show the positive bands of HSD17B4 but not control.
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