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HSD17B3 Antibody

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Western Blot: HSD17B3 Antibody [NBP2-16884] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HSD17B3 antibody diluted at 1:500.
Western Blot: HSD17B3 Antibody [NBP2-16884] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HSD17B3 antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: HSD17B3 Antibody [NBP2-16884] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: HSD17B3 protein stained by HSD17B3 antibody diluted at 1:500. Blue: Hoechst 33342 ...read more
Immunohistochemistry-Paraffin: HSD17B3 Antibody [NBP2-16884] - Human hepatoma, using HSD17B3 antibody antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Western Blot: HSD17B3 Antibody [NBP2-16884] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HSD17B3 antibody diluted at 1:500.
Western Blot: HSD17B3 Antibody [NBP2-16884] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSD17B3 antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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HSD17B3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human HSD17B3. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSD17B3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol (pH7).
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for HSD17B3 Antibody

  • EC 1.1.1.64
  • EDH17B317-beta-HSD3
  • hydroxysteroid (17-beta) dehydrogenase 3,17-beta-hydroxysteroid dehydrogenase type 3
  • SDR12C2
  • short chain dehydrogenase/reductase family 12C, member 2
  • Testicular 17-beta-hydroxysteroid dehydrogenase
  • testosterone 17-beta-dehydrogenase 3,17-beta-HSD 3

Background

This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 17A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 17A1.
Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151] - Staining in macaque Leydig cells. Image from a verified customer review.
Flow Cytometry: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Analysis of Jurkat cells, using anti-Cytochrome P450 17A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
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Western Blot: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NB100-1596] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aromatase Antibody NB100-1596 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol HSD17B3
Uniprot