HPGD Antibody

Images

 
Orthogonal Strategies: Western Blot: HPGD Antibody [NBP1-87061] - Analysis in human cell lines A-549 and A-431 using anti-HPGD antibody. Corresponding HPGD RNA-seq data are presented for the same cell lines. ...read more
Immunocytochemistry/ Immunofluorescence: HPGD Antibody [NBP1-87061] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Orthogonal Strategies: Immunohistochemistry-Paraffin: HPGD Antibody [NBP1-87061] - Staining in human urinary bladder and skeletal muscle tissues using anti-HPGD antibody. Corresponding HPGD RNA-seq data are ...read more
Western Blot: HPGD Antibody [NBP1-87061] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4
Immunohistochemistry-Paraffin: HPGD Antibody [NBP1-87061] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: HPGD Antibody [NBP1-87061] - Staining of human urinary bladder shows high expression.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

HPGD Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LAANLMNSGVRLNAICPGFVNTAILESIEKEENMGQYIEYKDHIKDMIKYYGI
Specificity
Specificity of human HPGD antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HPGD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1-4 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
HPGD Protein (NBP1-87061PEP)

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (81%), Rat (81%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HPGD Antibody

  • hydroxyprostaglandin dehydrogenase 15-(NAD)
  • NAD+-dependent 15-hydroxyprostaglandin dehydrogenase
  • Prostaglandin dehydrogenase 1
  • SDR36C1
  • Short Chain Dehydrogenase/Reductase Family 36C
  • short chain dehydrogenase/reductase family 36C, member 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HPGD Antibody (NBP1-87061) (0)

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Secondary Antibodies

 

Isotype Controls

Additional HPGD Products

HPGD NBP1-87061

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Bioinformatics

Gene Symbol HPGD