HP1 alpha Antibody

Images

 
Western Blot: HP1 alpha Antibody [NBP2-16876] - Sample (30 ug of whole cell lysate) A: HeLa B: HeLa nucleus 12% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: HP1 alpha Antibody [NBP2-16876] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HP1 alpha protein stained by HP1 alpha antibody diluted at 1:500. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

HP1 alpha Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human HP1 alpha. The exact sequence is proprietary.
Localization
Nucleus; Centromere
Marker
Heterochromatin Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CBX5
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HP1 alpha Antibody

  • Antigen p25
  • chromobox homolog 5 (Drosophila HP1 alpha)
  • chromobox homolog 5 (HP1 alpha homolog, Drosophila)
  • chromobox homolog 5
  • chromobox protein homolog 5
  • Heterochromatin protein 1 homolog alpha
  • heterochromatin protein 1-alpha
  • HP1 alpha homolog
  • HP1 alpha
  • HP1
  • HP1A
  • HP1-ALPHA
  • HP1Hs alpha
  • HP1Hs-alpha

Background

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HP1 alpha Antibody (NBP2-16876) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CBX5
Uniprot