HOXC11 Antibody (3E10)

Images

 
Western Blot: HOXC11 Antibody (3E10) [NBP2-00499] - Analysis of extracts (10ug) from 9 Human tissue by using anti-HOXC11 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: ...read more
Immunocytochemistry/ Immunofluorescence: HOXC11 Antibody (3E10) [NBP2-00499] Staining of COS7 cells transiently transfected by pCMV6-ENTRY HOXC11.
Immunohistochemistry-Paraffin: HOXC11 Antibody (3E10) [NBP2-00499] - Staining of paraffin-embedded Human tonsil using anti-HOXC11 mouse monoclonal antibody.
Flow Cytometry: HOXC11 Antibody (3E10) [NBP2-00499] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HOXC11 antibody, and then ...read more
Western Blot: HOXC11 Antibody (3E10) [NBP2-00499] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HOXC11 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Western Blot: HOXC11 Antibody (3E10) [NBP2-00499] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using antiHOXC11 monoclonal antibody.(1:200)
Immunohistochemistry-Paraffin: HOXC11 Antibody (3E10) [NBP2-00499] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HOXC11 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HOXC11 Antibody (3E10) [NBP2-00499] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HOXC11 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HOXC11 Antibody (3E10) [NBP2-00499] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HOXC11 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HOXC11 Antibody (3E10) [NBP2-00499] - Staining of paraffin-embedded Human endometrium tissue using anti-HOXC11 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
3E10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HOXC11 Antibody (3E10) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 1-304 of human HOXC11(NP_055027) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HOXC11
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:150

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HOXC11 Antibody (3E10)

  • homeo box C11
  • homeobox C11
  • Homeobox protein Hox-3H
  • homeobox protein Hox-C11
  • HOX3H
  • MGC4906

Background

This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXC genes located in a cluster on chromosome 12. The product of this gene binds to a promoter element of the lactase-phlorizin hydrolase. It also may play a role in early intestinal development. An alternatively spliced variant encoding a shorter isoform has been described but its full-length nature has not been determined. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HOXC11 Antibody (NBP2-00499). (Showing 1 - 1 of 1 FAQ).

  1. I have been using your HoxC11 3E10 primary for IHC staining and it interested to the epitope that is targeted by the Ab and also your IHC protocol you used to produce the images in particular the Antigen retrieval method.
    • The immunogen used to generate our HoxC11 antibody with catalogue number NBP2-00499 was a human recombinant protein fragment corresponding to amino acids 1-304 of human HOXC11 (NP_055027) produced in E.coli. We do not epitope map our antibodies and so, unfortunately, I am unable to provide you with any further information regarding the binding of this antibody to its target. Here is a link to our standard IHC-P protocol. Here is the antigen retrieval method, which uses sodium citrate buffer. Antigen Retrieval Solution: 0.01M Sodium Citrate Buffer, pH 6.0, To prepare stock solutions: Solution A. 0.1 M citric acid solution: dissolve 21.0 g of citric acid, monohydrate (C6H8O7.H2O) in 100 ml of dH2O. Solution B. 0.1M sodium citrate solution: dissolve 29.4 g trisodium citrate dihydrate (C6H5Na3O7.2H2O) in 100 ml of dH2O. Working solution: Add 9 ml of Stock solution A and 41 ml of stock solution B to 450 ml of dH2O. Adjust pH to 6.0. Antigen Retrieval: Pre-heat steamer or water bath with staining dish containing Sodium Citrate Buffer until temperature reaches 95-100 degrees Celsius. Immerse slides in the staining dish. Place the lid loosely on the staining dish and incubate for 20-40 minutes. Remove the staining dish to room temperature and allow the slides to cool for 20 minutes before proceeding with normal staining procedure.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HOXC11
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